文章：

miRNA-195及靶基因CDK6在口腔疣状癌中的研究

Research on miRNA-195 and target gene CDK6 in oral verrucous carcinoma 

原文发布日期：2017-06-16 

英文摘要：

Oral verrucous carcinoma (OVC) is a verrucous variant of oral cavity squamous carcinoma (OSCC). The expression of miRNA from OVC and OSCC including their matched normal oral mucosa tissues was profiled through the Affymetrix GeneChip miRNA Arrays. TargetScan and miRanda databases were used to predict the target gene of miRNA-195. The quantitative real-time PCR was applied to validate the expression of miRNA-195. The expression of CDK6 was investigated by the quantitative real-time PCR and immunohistochemistry. In this study, a total of 23 and 35 differentially expressed miRNAs were identified in OVC and OSCC, respectively. Moreover, 44 miRNAs were differentially expressed between OSCC and OVC. In addition, miRNA-195 was significantly decreased in both OVC and OSCC compared to normal oral mucosa. Target gene prediction demonstrated that CDK6 was a potential target gene of miRNA-195. In the quantitative real-time PCR, miR-195 was decreased in OVC and OSCC, which was consistent with the result of miRNA chip analysis. CDK6 was increased in OVC and OSCC, which was opposite to the expression of miRNA-195. In conclusion, miRNA-195 could be the potential diagnosis biomarker and therapy target of OVC. 

摘要翻译： 

口腔疣状癌（OVC）是口腔鳞状细胞癌（OSCC）的一种疣状变异亚型。本研究通过Affymetrix GeneChip miRNA芯片分析了OVC、OSCC及其配对正常口腔黏膜组织中的miRNA表达谱。采用TargetScan和miRanda数据库预测miRNA-195的靶基因，通过实时定量PCR验证miRNA-195的表达水平，并运用实时定量PCR和免疫组化技术检测CDK6的表达。结果显示：OVC中存在23个差异表达miRNA，OSCC中存在35个差异表达miRNA，而OSCC与OVC之间存在44个差异表达miRNA。与正常口腔黏膜相比，miRNA-195在OVC和OSCC中均显著下调。靶基因预测表明CDK6是miRNA-195的潜在靶基因。实时定量PCR证实miR-195在OVC和OSCC中表达降低，与miRNA芯片分析结果一致；而CDK6在OVC和OSCC中表达升高，与miRNA-195的表达趋势相反。结论表明：miRNA-195可能成为OVC潜在的诊断生物标志物和治疗靶点。

原文链接：

Research on miRNA-195 and target gene CDK6 in oral verrucous carcinoma