文章：

基于聚乙烯亚胺修饰二氧化硅纳米颗粒的有前途的基因传递系统

Promising gene delivery system based on polyethylenimine-modified silica nanoparticles

原文发布日期：2017-01-27

英文摘要：

This article reports on the synthesis and full characterization of innovative silica-based nanoparticle composed of fumed silica as a core decorated with polyethylenimine (PEI) with different molecular weights (25, 10 and 1.8 kDa). Wide range of analytical, spectroscopic, and microscopic methods (TEM, DLS, ζ potential, elemental analysis (EA), TNBS and FTIR) were used to characterize the nanoparticles. Furthermore, transfection efficiency of these nanoparticles as non-viral vector was examined. The silica-PEI conjugates retained both the ability of PEI to fully condense plasmid DNA at low N/P ratios and suitable buffering capacity at the endosomal pH range. PEI-functionalized silica remarkably enhanced EGFP-N1 gene expression in murine neuroblastoma (Neuro-2A) cells up to 38 folds compared to PEI 25 kDa. Meanwhile the results of the cytotoxicity assays indicated that these silica-PEI conjugates have acceptable level of viability.

摘要翻译：

本文报道了一种创新型二氧化硅基纳米粒的合成与全面表征：该纳米粒以气相二氧化硅为核心，表面修饰不同分子量（25、10及1.8 kDa）的聚乙烯亚胺（PEI）。研究采用多种分析学、光谱学及显微学方法（透射电镜、动态光散射、ζ电位、元素分析、TNBS法与傅里叶变换红外光谱）对纳米粒进行表征，并评估了其作为非病毒载体的转染效率。二氧化硅-PEI复合物既保留了PEI在低N/P比下完全缩合质粒DNA的能力，又具备在内体pH范围内的适宜缓冲容量。功能化后的二氧化硅-PEI可使增强型绿色荧光蛋白基因（EGFP-N1）在小鼠神经母细胞瘤（Neuro-2A）细胞中的表达量较PEI 25kDa对照组显著提升38倍，而细胞毒性实验表明该复合物具有可接受的生物相容性。

原文链接：

Promising gene delivery system based on polyethylenimine-modified silica nanoparticles