文章:

长链非编码RNA PRSS23-AS1作为ceRNA通过miR-3176/YBX1轴调控EMT促进乳腺癌进展

Long non-coding RNA PRSS23-AS1 as ceRNA promotes breast cancer progression by regulating EMT via miR-3176 /YBX1 axis 

原文发布日期：2025-07-24 

英文摘要：

Breast cancer (BC) remains a leading cause of cancer-related mortality, largely due to its aggressive proliferation and metastatic potential. Long non-coding RNAs (lncRNAs) have emerged as key regulators in tumor development and progression. This study explored the functional role and mechanism of Lnc-PRSS23-AS1 in BC. We assessed Lnc-PRSS23-AS1 expression and localization using fluorescence in situ hybridization, qRT-PCR, and Western blotting in BC tissues and cell lines. Binding interactions between Lnc-PRSS23-AS1, miR-3176, and Y-box binding protein 1 (YBX1) were validated through dual-luciferase reporter assays, RNA pulldown, and RNA immunoprecipitation. Lnc-PRSS23-AS1 was significantly upregulated in BC and predominantly localized in the cytoplasm. Silencing Lnc-PRSS23-AS1 or overexpressing miR-3176 suppressed BC cell proliferation, migration, and invasion in vitro and in vivo. Conversely, miR-3176 inhibition or YBX1 overexpression reversed these effects. Mechanistically, Lnc-PRSS23-AS1 promoted YBX1 protein expression by acting as a molecular sponge for miR-3176. These findings highlight the Lnc-PRSS23-AS1/miR-3176/YBX1 axis as a driver of BC progression and suggest Lnc-PRSS23-AS1 as a potential therapeutic target for breast cancer treatment. 

摘要翻译：

乳腺癌（BC）仍是癌症相关死亡的主要原因，这主要归因于其侵袭性增殖和转移潜能。长链非编码RNA（lncRNA）已成为肿瘤发生发展过程中的关键调控因子。本研究探讨了Lnc-PRSS23-AS1在乳腺癌中的功能作用及机制。我们通过荧光原位杂交、qRT-PCR和Western blotting技术检测了Lnc-PRSS23-AS1在乳腺癌组织和细胞系中的表达及定位；通过双荧光素酶报告基因实验、RNA pulldown和RNA免疫沉淀验证了Lnc-PRSS23-AS1与miR-3176、Y盒结合蛋白1（YBX1）之间的结合作用。Lnc-PRSS23-AS1在乳腺癌中显著上调且主要定位于细胞质。沉默Lnc-PRSS23-AS1或过表达miR-3176可在体内外抑制乳腺癌细胞增殖、迁移和侵袭；相反，抑制miR-3176或过表达YBX1可逆转这些效应。机制上，Lnc-PRSS23-AS1通过作为miR-3176的分子海绵促进YBX1蛋白表达。这些发现揭示了Lnc-PRSS23-AS1/miR-3176/YBX1轴是驱动乳腺癌进展的关键通路，表明Lnc-PRSS23-AS1可能成为乳腺癌治疗的潜在靶点。

原文链接：

Long non-coding RNA PRSS23-AS1 as ceRNA promotes breast cancer progression by regulating EMT via miR-3176 /YBX1 axis