文章：

LncRNA KCNQ1OT1通过靶向MiR-218-5p/HS3ST3B1促进膀胱癌的进展

LncRNA KCNQ1OT1 facilitates the progression of bladder cancer by targeting MiR-218-5p/HS3ST3B1 

原文发布日期：2020-08-20 

英文摘要：

Long non-coding RNA (lncRNA) is characterized by biological function in diverse cancers. LncRNA KCNQ1 opposite strand/antisense transcript 1 (KCNQ1OT1) is well acknowledged to regulate various cancers, while its role in bladder cancer remains unclear. In the present study, we aimed at probing into the impact and detailed mechanisms of KCNQ1OT1 in bladder cancer progression. In this study, we demonstrated that KCNQ1OT1 expression in bladder cancer tissues was notably up-regulated compared with in normal adjacent tissues, and KCNQ1OT1 modulated the malignant phenotypes of bladder cancer cells. Moreover, it was validated that KCNQ1OT1 could specifically bind to miR-218-5p and reduce its expression. Overexpressed miR-218-5p would inhibit the proliferation and metastasis of bladder cancer cells while facilitating apoptosis. In terms of Mechanism, Heparan Sulfate-Glucosamine 3-Sulfotransferase 3B1 (HS3ST3B1) was validated as a target gene of miR-218-5p, and could be regulated by KCNQ1OT1 indirectly. In conclusion, KCNQ1OT1 can promote the progression of bladder cancer through regulation of miR-218-5p/HS3ST3B1, which is expected to serve as a new therapeutic target for bladder cancer. 

摘要翻译： 

长链非编码RNA（lncRNA）在多种癌症中具有生物学功能特征。KCNQ1反向链/反义转录本1（KCNQ1OT1）被广泛认为可调控多种癌症，但其在膀胱癌中的作用尚不明确。本研究旨在探讨KCNQ1OT1在膀胱癌进展中的影响及具体作用机制。研究发现，膀胱癌组织中KCNQ1OT1的表达相较于癌旁正常组织显著上调，且KCNQ1OT1可调控膀胱癌细胞的恶性表型。进一步验证表明，KCNQ1OT1能特异性结合miR-218-5p并降低其表达水平。过表达miR-218-5p可抑制膀胱癌细胞增殖和转移，同时促进细胞凋亡。机制研究表明，硫酸乙酰肝素-葡萄糖胺3-磺基转移酶3B1（HS3ST3B1）是miR-218-5p的靶基因，并可被KCNQ1OT1间接调控。综上所述，KCNQ1OT1通过调控miR-218-5p/HS3ST3B1轴促进膀胱癌进展，这一机制有望成为膀胱癌治疗的新靶点。

原文链接：

LncRNA KCNQ1OT1 facilitates the progression of bladder cancer by targeting MiR-218-5p/HS3ST3B1