文章：

外泌体研究的最新进展：分离、表征与临床应用

Recent progress in exosome research: isolation, characterization and clinical applications

原文发布日期：2023-04-27 

英文摘要：

Exosomes, a kind of nano-vesicles released by various cell types, carry a variety of “cargos” including proteins, RNAs, DNAs and lipids. There is substantial evidence that exosomes are involved in intercellular communication by exchanging “cargos” among cells and play important roles in cancer development. Because of the different expressions of “cargos” carried by exosomes in biological fluids under physiological and pathological conditions, exosomes have the potential as a minimally invasive method of liquid biopsy for cancer diagnosis and prognosis. In addition, due to their good biocompatibility, safety, biodistribution and low immunogenicity, exosomes also have potential applications in the development of promising cancer treatment methods. In this review, we summarize the recent progress in the isolation and characterization techniques of exosomes. Moreover, we review the biological functions of exosomes in regulating tumor metastasis, drug resistance and immune regulation during cancer development and outline the applications of exosomes in cancer therapy. 

摘要翻译： 

外泌体是多种细胞释放的一种纳米级囊泡，携带有蛋白质、RNA、DNA及脂质等多种"货物"。大量证据表明，外泌体通过介导细胞间"货物"交换参与细胞通讯，在癌症发展中发挥重要作用。由于生理和病理状态下生物体液中外泌体所携带"货物"的表达存在差异，使其具备作为微创液体活检手段用于癌症诊断与预后评估的潜力。此外，得益于良好的生物相容性、安全性、生物分布特性及低免疫原性，外泌体在开发新型癌症治疗方法中也具有应用前景。本文综述了外泌体分离与表征技术的最新进展，阐述了外泌体在癌症发展中调控肿瘤转移、耐药性及免疫调节的生物学功能，并概述了外泌体在癌症治疗中的应用。

原文链接：

Recent progress in exosome research: isolation, characterization and clinical applications