文章：

抑制microRNA-384-5p可通过

Inhibition of microRNA-384-5p alleviates osteoarthritis through 

原文发布日期：2018-07-30 

英文摘要：

Osteoarthritis (OA), a major cause of pain and disability, is a serious public issue worldwide. Some microRNAs (miRNAs) and SOX9 have been found to be expressed in OA. Therefore, the aim of this study is to investigate effects of microRNA-384-5p (miR-384-5p) on cartilage cell proliferation and apoptosis in mice with OA by targeting SOX9 through the NF-κB signaling pathway. First, bioinformatics was used to predict the SOX9-mediated miRNA (miR-384-5p), and dual luciferase reporter gene assay was conducted to further verify the relationship between miR-384-5p and SOX9. Then, the expression of miR-384-5p, SOX9, and NF-kB in mice modeled with OA was detected. To investigate the specific mechanism of miR-384-5p in OA, mimic and inhibitor of miR-384-5p and siRNA against SOX9 were used to transfect cartilage cells. Finally, proliferation, cell cycle, and cell apoptosis were detected using MTT assay and flow cytometry, respectively. Our results indicated that OA mice exhibited decreased expression of SOX9 and NF-kB but higher miR-384-5p expression. In addition, over-expressed miR-384-5p or silenced SOX9 could inhibit
cell proliferation, and block cell cycle entry and induces apoptosis. SOX9 was a target gene of miR-384-5p. The NF-kB signaling pathway was inactivated after overexpression of miR-384-5p. Furthermore, we also observed that the effect of miR-384-5p inhibition was rescued when SOX9 was knocked down. The results support the view that inhibition of miR-384-5p could impede apoptosis and promote proliferation of cartilage cells through activating the NF-κB signaling pathway by promoting SOX9, thereby preventing the development of OA.

摘要翻译：

骨关节炎（OA）是导致疼痛和功能障碍的主要病因，已成为全球重要的公共卫生问题。研究发现某些微小RNA（miRNA）及SOX9在OA中存在特异性表达。本研究旨在通过NF-κB信号通路靶向SOX9，探讨microRNA-384-5p（miR-384-5p）对OA小鼠软骨细胞增殖与凋亡的影响。首先采用生物信息学预测SOX9介导的miRNA（miR-384-5p），并通过双荧光素酶报告基因实验验证两者靶向关系；检测OA模型小鼠中miR-384-5p、SOX9及NF-κB的表达水平；为探究miR-384-5p在OA中的具体作用机制，分别用miR-384-5p模拟物、抑制剂及SOX9 siRNA转染软骨细胞；最后通过MTT法和流式细胞术分别检测细胞增殖、周期及凋亡。结果显示：OA小鼠SOX9和NF-κB表达降低，miR-384-5p表达升高；过表达miR-384-5p或沉默SOX9可抑制细胞增殖、阻滞细胞周期并诱导凋亡；SOX9被证实是miR-384-5p的靶基因；过表达miR-384-5p会抑制NF-κB信号通路活性；此外还发现敲低SOX9可逆转抑制miR-384-5p产生的保护效应。研究结果表明：抑制miR-384-5p可通过上调SOX9激活NF-κB信号通路，从而阻碍软骨细胞凋亡、促进增殖，最终延缓OA进程。

原文链接：

Inhibition of microRNA-384-5p alleviates osteoarthritis through