文章：

GTF2E2被miR-340-5p下调抑制胶质母细胞瘤的恶性进展

GTF2E2 downregulated by miR-340-5p inhibits the malignant progression of glioblastoma 

原文发布日期：2023-10-16 

英文摘要：

Glioblastoma is the most common malignant tumor in the central nervous system. The general transcription factor IIE subunit beta (GTF2E2) is crucial for physiological and pathological functions, but its roles in the malignant biological function of glioma remain ambiguous. CCK-8, colony formation assays, TUNEL assays, cell migration assays, wound-healing assays, and xenograft model were established to investigate the biological functions of GTF2E2 both in vitro and in vivo. GTF2E2 was overexpressed in glioma and was associated with poor prognosis of glioma patients. Biological functions of GTF2E2 were investigated both in vitro and in vi0vo by multiple experiments. Moreover, we explored the possible mechanisms of GTF2E2. In our results, we demonstrated that GTF2E2 could be regulated by miR-340-5p directly or indirectly. CCND1 was transcriptionally affected by GTF2E2 and glioma progression was then regulated. Our data presented the overexpression of GTF2E2 in glioma and indicated the association between GTF2E2 and glioma prognosis. GTF2E2 was found to be regulated by miR-340-5p and thus affect downstream gene expressions and glioma progression. Our results indicate that GTF2E2 might be a potential target in the diagnosis and treatments of glioblastoma. 

摘要翻译：

胶质母细胞瘤是中枢神经系统中最常见的恶性肿瘤。通用转录因子IIEβ亚基（GTF2E2）在生理和病理功能中起关键作用，但其在胶质瘤恶性生物学功能中的作用尚不明确。本研究通过CCK-8实验、集落形成实验、TUNEL检测、细胞迁移实验、伤口愈合实验以及异种移植模型，从体外和体内两个层面探究了GTF2E2的生物学功能。研究发现GTF2E2在胶质瘤中过表达，且与患者不良预后相关。通过多组实验证实，GTF2E2在体外和体内均表现出促癌特性。进一步机制研究表明，GTF2E2可直接或间接受miR-340-5p调控，并通过转录调控CCND1影响胶质瘤进展。本研究表明GTF2E2在胶质瘤中过表达且与预后相关，其受miR-340-5p调控并影响下游基因表达及肿瘤进展，提示GTF2E2可能成为胶质母细胞瘤诊断和治疗的潜在靶点。

原文链接：

GTF2E2 downregulated by miR-340-5p inhibits the malignant progression of glioblastoma