文章：

DRAIC通过海绵miR-432-5p上调SLBP来促进乳腺癌的生长

DRAIC promotes growth of breast cancer by sponging miR-432-5p to upregulate SLBP 

原文发布日期：2021-10-13 

英文摘要：

Mounting evidence suggests that lncRNAs can exert functions in cancer progression in multiple manners. In recent years, competing endogenous RNA (ceRNA) has been widely reported in human cancers as a lncRNA-dominant molecular pathway. The current study aimed at proving the role of lncRNA downregulated RNA in cancer (DRAIC) in breast cancer (BRCA) progression. To be specific, qRT-PCR assay was conducted to measure the expression of DRAIC and other downstream target genes. It was uncovered that DRAIC was expressed at a high level in BRCA cells. Functional analyses, including CCK-8, colony formation, and EdU assays demonstrated that DRAIC depletion suppressed BRCA cell proliferation. In addition, cell apoptosis was promoted due to DRAIC knockdown. The inhibitory effect of DRAIC reduction on BRCA cell migration and invasion was proven by transwell assays. Mechanistically, DRAIC was confirmed to predominantly distribute in the cytoplasm and could interact with miR-432-5p. In addition, stem-loop binding protein (SLBP) was verified to be a downstream target of miR-432-5p and was positively regulated by DRAIC. Taken together, DRAIC sponged miR-432-5p to enhance SLBP expression, by which malignant behaviors of BRCA cells were promoted. Our findings may help to provide a promising therapeutic target for BRCA patients. 

摘要翻译：

越来越多的证据表明，长链非编码RNA（lncRNA）能以多种方式参与癌症进展。近年来，竞争性内源RNA（ceRNA）作为lncRNA主导的分子通路在人类癌症中得到广泛报道。本研究旨在证实lncRNA DRAIC在乳腺癌（BRCA）进展中的作用。具体通过qRT-PCR检测发现DRAIC在乳腺癌细胞中高表达。CCK-8、集落形成和EdU实验等功能分析表明，敲低DRAIC可抑制乳腺癌细胞增殖并促进细胞凋亡。Transwell实验进一步证实DRAIC下调对乳腺癌细胞迁移和侵袭的抑制作用。机制研究发现，DRAIC主要定位于细胞质，并可结合miR-432-5p；茎环结合蛋白（SLBP）被验证为miR-432-5p的下游靶标，且受DRAIC正向调控。综上，DRAIC通过吸附miR-432-5p增强SLBP表达，从而促进乳腺癌细胞的恶性行为。本研究可能为乳腺癌患者提供潜在的治疗靶点。

原文链接：

DRAIC promotes growth of breast cancer by sponging miR-432-5p to upregulate SLBP