文章：

脱细胞的正常组织和癌组织作为癌症研究的工具

Decellularized normal and cancer tissues as tools for cancer research 

原文发布日期：2021-11-16 

英文摘要：

Today it is widely accepted that molecular mechanisms triggering cancer initiate with a genetic modification. However, a genetic alteration providing the aberrant clone with a growing advantage over neighboring cells is not sufficient to develop cancer. Currently, tumors are considered a heterogeneous population of cells and an extracellular matrix (ECM) that make up a characteristic microenvironment. Interactions between tumor cells and cancer microenvironment define cancer progression and therapeutic response. To investigate and clarify the role of ECM in the regulation of cancer cell behavior and response to therapy, the decellularization of ECM, a widely used technique in tissue engineering, has been recently employed to develop 3D culture model of disease. In this review, we briefly explore the different components of healthy and pathological ECM and the methods to obtain and characterize the ECM from native bioptic tissue. Finally, we highlight the most relevant applications of ECM in translational cancer research strategies: decellularized ECM, ECM-hydrogel and 3D bioprinting. 

摘要翻译： 

如今，人们普遍认为触发癌症的分子机制始于基因改变。然而，仅凭赋予异常克隆相较于邻近细胞生长优势的基因改变并不足以形成癌症。当前，肿瘤被视为由异质性细胞群和细胞外基质（ECM）组成的特殊微环境。肿瘤细胞与癌症微环境之间的相互作用决定了癌症的进展和治疗反应。为研究并阐明ECM在调控癌细胞行为及治疗反应中的作用，组织工程中广泛应用的ECM脱细胞技术近期被用于开发疾病的三维培养模型。本综述简要探讨了健康与病理状态下ECM的不同组成成分，以及从原生活检组织中获取并表征ECM的方法，最后重点介绍了ECM在转化性癌症研究策略中最具相关性的应用：脱细胞ECM、ECM水凝胶和3D生物打印技术。

原文链接：

Decellularized normal and cancer tissues as tools for cancer research