文章：

CRISPR/Cas9基因组编辑技术显著加速了单纯疱疹病毒的研究

CRISPR/Cas9 genome editing technology significantly accelerated herpes simplex virus research 

原文发布日期：2018-04-25 

英文摘要：

Herpes simplex viruses (HSVs) are important pathogens and ideal for gene therapy due to its large genome size. Previous researches on HSVs were hampered because the technology to construct recombinant HSVs were based on DNA homology-dependent repair (HDR) and plaque assay, which are inefficient, laborious, and time-consuming. Fortunately, clustered regularly interspaced short palindromic repeat/CRISPR-associated protein 9 (CRISPR/Cas9) recently provided the possibility to precisely, efficiently, and rapidly edit genomes and indeed is successfully being used in HSVs. Importantly, CRISPR/Cas9 technology increased HSV HDR efficiency exponentially by a 10,000–1,000,000 times when making recombinant HSVs, and its combination with flow cytometric technology made HSV recombination practically automatic. These may have a significant impact on virus and gene therapy researches. This review will summarize the latest development and molecular mechanisms of CRISPR/Cas9 genome editing technology and its recent application in HSVs. 

摘要翻译： 

单纯疱疹病毒（HSV）是一种重要病原体，因其基因组庞大而成为基因治疗的理想载体。以往基于DNA同源定向修复（HDR）和噬斑试验的重组HSV构建技术存在效率低下、操作繁琐且耗时等问题，严重制约了HSV相关研究的进展。值得庆幸的是，成簇规律间隔短回文重复序列及其相关蛋白9（CRISPR/Cas9）技术的出现，为精准、高效、快速的基因组编辑提供了可能，并已成功应用于HSV研究。尤为重要的是，CRISPR/Cas9技术将重组HSV的HDR效率提升了10,000至1,000,000倍，且与流式细胞术联用实现了HSV重组过程的近乎自动化。这些突破性进展将对病毒学及基因治疗研究领域产生深远影响。本文综述了CRISPR/Cas9基因组编辑技术的最新进展、分子机制及其在HSV研究中的前沿应用。

原文链接：

CRISPR/Cas9 genome editing technology significantly accelerated herpes simplex virus research