文章：

circPTPN22通过STAT3乙酰化减弱胰腺癌免疫微环境

circPTPN22 attenuates immune microenvironment of pancreatic cancer via STAT3 acetylation 

原文发布日期：2021-09-01 

英文摘要：

Accumulating research implicated that circular RNAs exhibited significant roles in cancer development. Nonetheless, the role regarding circPTPN22 in pancreatic cancer remains unclear. Expression of circPTPN22 in pancreatic cancer cell lines and normal cells was determined with quantitative real-time PCR (qRT-PCR). Cell counting kit-8 assay and colony formation assay were used to measure the proliferation of pancreatic cancer cells. RNA immunoprecipitation and Western blot were employed for investigation the binding between circPTPN22 and STAT3. circPTPN22 expression was highly upregulated in pancreatic cancer tissues and cell lines. Knockdown of circPTPN22 inhibited cell proliferation and attenuates pancreatic cancer immune microenvironment. Furthermore, STAT3 acetylation was involved in these effects. circPTPN22 promoted STAT3 acetylation via inhibiting STAT3/SIRT1 interaction. circPTPN22 attenuates pancreatic cancer immune microenvironment by promoting STAT3 acetylation via inhibiting STAT3/SIRT1 interaction. 

摘要翻译：

不断涌现的研究表明，环状RNA在癌症发展中发挥着重要作用。然而，circPTPN22在胰腺癌中的作用尚不明确。本研究通过实时荧光定量PCR（qRT-PCR）检测了circPTPN22在胰腺癌细胞系和正常细胞中的表达水平。采用细胞计数试剂盒-8（CCK-8）实验和集落形成实验评估胰腺癌细胞的增殖能力。通过RNA免疫共沉淀和Western blot技术探究circPTPN22与STAT3的结合关系。结果显示，circPTPN22在胰腺癌组织和细胞系中表达显著上调。敲低circPTPN22可抑制细胞增殖并改善胰腺癌免疫微环境。进一步机制研究表明，STAT3乙酰化参与这一过程——circPTPN22通过抑制STAT3/SIRT1相互作用促进STAT3乙酰化。最终证实，circPTPN22通过阻断STAT3/SIRT1相互作用促进STAT3乙酰化，从而改善胰腺癌免疫微环境。

原文链接：

circPTPN22 attenuates immune microenvironment of pancreatic cancer via STAT3 acetylation