文章：

用基因增强的T淋巴细胞靶向肿瘤

Targeting tumours with genetically enhanced T lymphocytes

原文发布日期：2003-01-01

DOI: 10.1038/nrc971

类型: Review Article

开放获取: 否

要点：

1. The genetic modification of T lymphocytes is the basis for novel approaches to study tumour immunity.
2. Antigen specificity can be redirected by the transduction of physiological and chimeric antigen receptors into T cells.
3. T-cell proliferation and function can be increased by genetically manipulating cells to express modified co-stimulatory receptors.
4. T cells can be genetically protected from immunosuppressive factors such as transforming growth factor-β.
5. T cells can also be genetically modified to include regulatable suicide mechanisms, thereby ensuring the safety of adoptive-cell therapy.
6. Expressing constitutive or inducible markers in T cells allows the tracking of cell migration and activation using non-invasive imaging technologies.

要点翻译：

1. T淋巴细胞的基因改造是研究肿瘤免疫新策略的基础。
2. 通过向T细胞转导生理性和嵌合抗原受体，可重定向其抗原特异性。
3. 通过基因操作使细胞表达修饰后的共刺激受体，可增强T细胞的增殖与功能。
4. 通过基因改造可使T细胞免受转化生长因子-β等免疫抑制因子的影响。
5. T细胞还可被改造为包含可调控的"自杀机制"，从而确保过继细胞疗法的安全性。
6. 在T细胞中表达组成型或诱导型标记物，可通过无创成像技术追踪细胞迁移与活化过程。

英文摘要：

The genetic modification of T lymphocytes is an important approach to investigating normal T-cell biology and to increasing antitumour immunity. A number of genetic strategies aim to increase the recognition of tumour antigens, enhance antitumour activities and prevent T-cell malfunction. T cells can also be engineered to increase safety, as well as to express markers that can be tracked by non-invasive imaging technologies. Genetically modified T cells are therefore proving to be of great value for basic immunology and experimental immunotherapy.

摘要翻译： 

T淋巴细胞的基因修饰是研究正常T细胞生物学和增强抗肿瘤免疫力的重要手段。多种基因策略旨在提高对肿瘤抗原的识别、增强抗肿瘤活性并防止T细胞功能异常。T细胞还可被工程化以增强安全性，并表达可通过非侵入性影像技术追踪的标志物。因此，基因修饰的T细胞在基础免疫学和实验性免疫治疗中显示出重要价值。

原文链接：

Targeting tumours with genetically enhanced T lymphocytes