文章：

常见的增殖标志物

Common markers of proliferation

原文发布日期：2006-02-01

DOI: 10.1038/nrc1802

类型: Review Article

开放获取: 否

要点：

1. The genes that are most often found when tumour and normal tissues are compared by gene expression are those involved in proliferation.
2. Increased expression of the proliferation genes in tumours is often associated with poor prognoses in cancer patients.
3. Cell-cycle-regulated genes have been identified by microarray analysis of cells in culture. Contained in this list are genes that are expressed in each cell cycle phase: G1/S, S, G2 and M phases.
4. Comparing the proliferation genes found in tumours and cell-cycle-regulated genes found in cultured cells in vitro shows that there is a significant overlap between these two signatures.
5. The cell-cycle-regulated genes identified by microarray analysis provide biomarkers of proliferation in both normal cells and tumours. The observation that the proliferation signature is so clearly identifiable indicates that it could be a component of genomic-based clinical diagnostics for cancer patients.

要点翻译：

1. 在肿瘤与正常组织的基因表达比较中，最常发现的基因是与增殖相关的基因。
2. 肿瘤中增殖基因表达增强常预示癌症患者预后不良。  
3. 通过培养细胞的微阵列分析已识别出细胞周期调控基因。该基因列表包含在各细胞周期阶段（G1/S期、S期、G2期和M期）表达的基因。  
4. 对比肿瘤中的增殖基因与体外培养细胞发现的细胞周期调控基因，可见这两种特征基因存在显著重叠。  
5. 微阵列分析鉴定的细胞周期调控基因可作为正常细胞和肿瘤的增殖生物标志物。增殖特征能如此清晰识别表明，它可能成为癌症患者基因组临床诊断的组成部分。

英文摘要：

When normal tissue and tumour samples are compared by microarray analysis, the biggest differences most often occur in the expression levels of genes that control cell proliferation. However, this difference is detected whenever mRNA samples that are taken from two cell populations with different proliferation rates are compared. Although the exact genes that comprise this 'proliferation signature' often differ, they are almost always genes that are involved in the fundamental process of cell proliferation. Can the proliferation signature be used to improve our understanding of the cell cycle and cancer pathogenesis, as well as being used as a biomarker for cancer diagnosis and prognosis?

摘要翻译： 

当通过微阵列分析比较正常组织与肿瘤样本时，最显著的差异通常出现在调控细胞增殖的基因表达水平上。然而，只要比较来自两个增殖速率不同的细胞群体的mRNA样本，就能检测到这种差异。尽管构成这一“增殖特征”的具体基因常常不同，它们几乎总是参与细胞增殖这一基本过程的基因。增殖特征能否用于加深我们对细胞周期和癌症发病机制的理解，并作为癌症诊断和预后的生物标志物？

原文链接：

Common markers of proliferation