文章：

三维培养中腺上皮癌的建模

Modelling glandular epithelial cancers in three-dimensional cultures

原文发布日期：2005-09-01

DOI: 10.1038/nrc1695

类型: Review Article

开放获取: 否

要点：

1. Epithelial cells grown in 3D cultures recapitulate numerous features of the glandular epithelium in vivo. These include the formation of cyst-like spheroids with a hollow lumen, apicobasal polarization of the cells comprising these structures, tight control of cell growth and proliferation, and the establishment of a basement membrane.
2. Filling of the lumen with cells is a salient feature of early glandular epithelial cancers that remains poorly understood. Studies in 3D cultures have uncovered multiple processes and regulatory molecules that are involved in the formation and maintenance of luminal space.
3. Known cancer genes give rise to a diverse array of morphogenetic phenotypes in 3D cultures that resemble important histopathological features observed in epithelial cancers in vivo.
4. Apicobasal cell polarity is a fundamental characteristic of the glandular epithelium both in vivo and in vitro. Recent studies in 3D systems illustrate the importance of this process for an intact architecture.
5. Various invasive properties have been observed in 3D systems, including invasion through the basement membrane. Future efforts directed towards co-culture systems that more faithfully represent the histological complexity of epithelial tissue in vivo are crucial for our understanding of epithelial cancers.

要点翻译：

1. 在三维培养体系中生长的上皮细胞，能够重现体内腺上皮的诸多特征。这些特征包括形成具有中空腔隙的囊状球体、构成这些结构的细胞出现顶基底极化、细胞生长与增殖受到严格调控，以及基底膜的建立。
2. 腔隙被细胞填充是早期腺上皮癌的显著特征，其机制至今仍未明确。三维培养研究揭示了参与腔隙形成与维持的多种过程及调控分子。
3. 已知癌症基因在三维培养中可产生多种形态发生表型，这些表型与体内上皮癌组织的重要病理学特征高度相似。
4. 顶基底细胞极性是腺上皮在体内和体外的核心特征。近期三维系统研究表明，这一过程对维持完整组织结构至关重要。
5. 在三维系统中已观察到多种侵袭特性，包括穿透基底膜的侵袭行为。未来致力于开发更真实模拟体内上皮组织复杂结构的共培养体系，对深入理解上皮癌具有重要意义。

英文摘要：

Little is known about how the genotypic and molecular abnormalities associated with epithelial cancers actually contribute to the histological phenotypes observed in tumours in vivo. 3D epithelial culture systems are a valuable tool for modelling cancer genes and pathways in a structurally appropriate context. Here, we review the important features of epithelial structures grown in 3D basement membrane cultures, and how such models have been used to investigate the mechanisms associated with tumour initiation and progression.

摘要翻译： 

目前，关于上皮癌中基因型和分子异常如何具体导致体内肿瘤组织学表型的机制仍知之甚少。三维上皮培养系统是一种在结构适宜的环境中模拟癌症基因和通路的有效工具。本文综述了在三维基底膜培养中形成的上皮结构的重要特征，并探讨了此类模型如何被用于研究肿瘤发生与进展的相关机制。

原文链接：

Modelling glandular epithelial cancers in three-dimensional cultures