文章：

利用快速半自动化流式细胞术预测直肠癌放化疗反应

Response Prediction of Chemoradiotherapy for Rectal Cancer Using Rapid Semi-Automated Flow Cytometry

原文发布日期：19 December 2025

DOI: 10.3390/cancers18010001

类型: Article

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英文摘要：

(1) Background: The effectiveness of chemoradiotherapy (CRT) for rectal cancer varies greatly among patients, and no reliable biomarker for predicting treatment response currently exists. We investigated whether cell cycle analysis using rapid semi-automated flow cytometry could predict the effectiveness of CRT. (2) Methods: This prospective study included 32 patients with rectal cancer undergoing CRT. The cell cycle of biopsy specimens from rectal cancer patients before CRT was semi-automatically and rapidly analyzed using Celltac PEAK as flow cytometer. The proportion of cell cycle phases is compared with treatment response after CRT. (3) Results: Patients with a tumor reduction rate ≥ 30% on CT showed a significantly higher proportion of cells in the Over G2/M phase compared with those with <30% reduction (median 2.45% vs. 0.95%,p= 0.022). Similarly, tumors demonstrating disappearance of FDG uptake after CRT had a significantly higher proportion of cells in the Over G2/M phase compared with those with persistent FDG uptake (median 4.05% vs. 1.24%,p= 0.024). (4) Conclusions: A high proportion of cells in the Over G2/M phase could be a potential biomarker for predicting tumor shrinkage in CRT for rectal cancer.

摘要翻译： 

（1）背景：直肠癌患者对放化疗（CRT）的疗效反应存在显著个体差异，目前尚无可靠的生物标志物可用于预测治疗效果。本研究探讨了基于快速半自动化流式细胞术的细胞周期分析能否预测CRT疗效。（2）方法：这项前瞻性研究纳入了32例接受CRT的直肠癌患者。采用Celltac PEAK流式细胞仪对患者CRT前活检标本进行半自动化快速细胞周期分析。将细胞周期各时相比例与CRT后的治疗反应进行对比。（3）结果：CT显示肿瘤缩小率≥30%的患者，其细胞周期中Over G2/M期细胞比例显著高于缩小率<30%的患者（中位数2.45% vs. 0.95%，p=0.022）。同样，CRT后FDG摄取消失的肿瘤组织，其Over G2/M期细胞比例显著高于FDG摄取持续存在的肿瘤（中位数4.05% vs. 1.24%，p=0.024）。（4）结论：Over G2/M期细胞高比例可能成为预测直肠癌放化疗肿瘤缩小的潜在生物标志物。

原文链接：

Response Prediction of Chemoradiotherapy for Rectal Cancer Using Rapid Semi-Automated Flow Cytometry