Background/Objective:Cervical cancer incidence is rising in Puerto Rico (PR) despite being preventable through HPV vaccination, Pap smear screening, and the timely treatment of dysplasia. This study evaluated the presence of single-nucleotide polymorphisms (SNPs) in Toll-like receptors (TLR4 and TLR9) among women with cervical dysplasia and HPV infection, as potential immune-related susceptibility factors.Methods:Cervicovaginal samples from 210 Hispanic women in PR were analyzed for dysplasia severity and HPV genotype. Demographic data were collected. Seven SNPs (four in TLR4, three in TLR9) were examined using PCR-RFLP and AS-PCR. Associations between SNP frequency, dysplasia severity, and high-risk HPV (HR-HPV) were assessed.Results:HR-HPV was found in 64% of severe dysplasia cases versus 46% of mild/negative cases (p< 0.025). The TLR4 AG (rs4986790) and CT (rs1927911) genotypes were more frequent in severe dysplasia. For TLR9, CT heterozygotes (rs187084) and the TC genotype (rs5743836) were also enriched in severe dysplasia.Conclusions:This first PR-based study shows that TLR4 and TLR9 SNPs significantly correlate with HR-HPV infection and dysplasia severity, supporting further research on their potential as biomarkers for cervical cancer prevention.
背景/目的：尽管可通过人乳头瘤病毒（HPV）疫苗接种、巴氏涂片筛查及不典型增生的及时治疗进行预防，但波多黎各（PR）的宫颈癌发病率仍在上升。本研究评估了宫颈不典型增生合并HPV感染女性中Toll样受体（TLR4和TLR9）单核苷酸多态性（SNPs）的存在情况，探讨其作为潜在免疫相关易感因素的可能性。 方法：对210名波多黎各西班牙裔女性的宫颈阴道样本进行不典型增生严重程度和HPV基因型分析，并收集人口统计学数据。采用PCR-RFLP和AS-PCR技术检测7个SNPs（TLR4基因4个，TLR9基因3个），评估SNP频率与不典型增生严重程度及高危型HPV（HR-HPV）感染之间的关联。 结果：重度不典型增生病例中HR-HPV检出率为64%，而轻度/阴性病例中为46%（p<0.025）。TLR4基因AG（rs4986790）和CT（rs1927911）基因型在重度不典型增生中更常见。TLR9基因CT杂合子（rs187084）和TC基因型（rs5743836）同样在重度不典型增生中富集。 结论：这项首次基于波多黎各人群的研究表明，TLR4和TLR9基因SNPs与HR-HPV感染及不典型增生严重程度显著相关，支持进一步研究其作为宫颈癌预防生物标志物的潜力。
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