Background: Physical plasma, the fourth state of matter formed through gas ionization, has shown promise in various clinical applications, including wound healing and antimicrobial therapy. Recently, Non-invasive physical plasma (NIPP) selectively disrupts tumor cell proliferation and metabolism without inducing cytoprotective stress responses, positioning it as a promising adjunct in oncological therapies, though its underlying mechanisms remain insufficiently understood.Methods: In this study, we investigated the effects of NIPP (Plasma Care device) on six tumor cell lines, ovarian (SKOV-3, OVCAR-3), prostate (LNCaP, PC-3), and breast (MCF-7, MDA-MB-231). Cell proliferation and migration were assessed using CASY analysis and scratch assays, while cytoskeletal integrity, heat shock protein (HSP) expression, and key metabolic indicators were evaluated through immunofluorescence, Western blotting, and biochemical assays.Results: NIPP treatment significantly inhibited tumor cell proliferation and migration, disrupted cytoskeletal organization, and altered metabolic activity in a time-dependent manner. These effects were associated with increased intracellular reactive oxygen species (ROS), decreased mitochondrial membrane potential (MMP), enhanced glycolysis, and elevated lactate production. Notably, despite cellular stress, neither HSP expression nor superoxide dismutase (SOD) activity showed significant changes, suggesting a lack of classical stress-response activation.Conclusions: Our findings indicate that NIPP selectively impairs tumor cell function by inducing oxidative stress and metabolic disruption, without triggering protective HSP-mediated resistance pathways commonly seen in radiotherapy and chemotherapy. These results highlight the therapeutic potential of NIPP, particularly via the Plasma Care device, as a novel anticancer strategy.
背景：物理等离子体作为气体电离形成的第四态物质，在伤口愈合和抗菌治疗等多种临床应用中展现出潜力。近期研究发现，非侵入性物理等离子体能够选择性破坏肿瘤细胞的增殖与代谢，且不诱发细胞保护性应激反应，这使其成为肿瘤治疗中极具前景的辅助手段，但其作用机制尚未得到充分阐释。 方法：本研究采用等离子体护理设备产生的非侵入性物理等离子体，对六种肿瘤细胞系（卵巢癌SKOV-3、OVCAR-3，前列腺癌LNCaP、PC-3，乳腺癌MCF-7、MDA-MB-231）进行处理。通过CASY细胞分析系统和划痕实验评估细胞增殖与迁移能力；运用免疫荧光、Western印迹及生化检测技术分析细胞骨架完整性、热休克蛋白表达及关键代谢指标。 结果：非侵入性物理等离子体处理能显著抑制肿瘤细胞增殖与迁移，破坏细胞骨架结构，并以时间依赖性方式改变细胞代谢活性。这些效应与细胞内活性氧水平升高、线粒体膜电位降低、糖酵解增强及乳酸产量增加密切相关。值得注意的是，尽管细胞处于应激状态，热休克蛋白表达与超氧化物歧化酶活性均未发生显著变化，提示经典应激反应通路未被激活。 结论：本研究表明非侵入性物理等离子体通过诱导氧化应激和代谢紊乱选择性损伤肿瘤细胞功能，且未触发放疗和化疗中常见的由热休克蛋白介导的保护性耐药通路。这些发现凸显了非侵入性物理等离子体（特别是通过等离子体护理设备实施）作为新型抗癌策略的治疗潜力。
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