Objectives:MicroRNAs of themiR-200family are recognized as key inhibitors of epithelial-to-mesenchymal transition (EMT). However, there is limited data on the potential regulation ofmiR-200family expression by long non-coding RNAs (lncRNAs) in RCC.Methods:We conducted a comprehensive literature and database search to identify lncRNAs that had been already functionally validated as regulators of any member of themiR-200family. We analyzed the expression levels of themiR-200family and the identified lncRNAs by qPCR. The study included 42 samples of carcinoma and non-carcinoma tissue from 25 RCC patients. In addition, we used RNA sequencing data from The Cancer Genome Atlas (TCGA), encompassing 511 kidney RCC (KIRC) samples, to further analyze the expression of miRNAs and lncRNAs.Results:We identified 127 lncRNAs with confirmed regulatory functions, 31 of which were validated in our samples. The majority of lncRNAs, along with all members of themiR-200family, showed consistent downregulation in carcinoma tissues compared to non-carcinoma tissues. We observed a significant correlation between the expression of at least one member of themiR-200family and 17 lncRNAs. In particular, three lncRNAs (MALAT1,OIP5-AS1, andLINC00467)showed a correlation with the expression of all members of themiR-200family. Our results were at least partially confirmed in KIRC samples from the TCGA dataset.Conclusions:Our results suggest that the expression of themiR-200family in RCC might be at least partially influenced by lncRNAs. Based on our cohort of samples,MALAT1,OIP5-AS1, andLINC00467appear to be potentially important contributors to RCC development.
目的：miR-200家族microRNA被认为是上皮-间质转化（EMT）的关键抑制因子。然而，关于长链非编码RNA（lncRNA）在肾细胞癌（RCC）中可能调控miR-200家族表达的数据有限。方法：我们进行了系统的文献和数据库检索，以识别已被功能验证为miR-200家族任何成员调控因子的lncRNAs。通过qPCR分析了miR-200家族及已识别lncRNAs的表达水平。本研究纳入了25名RCC患者的42份癌组织与非癌组织样本。此外，我们利用癌症基因组图谱（TCGA）中511例肾透明细胞癌（KIRC）样本的RNA测序数据，进一步分析了miRNA和lncRNA的表达情况。结果：我们识别出127个具有已确认调控功能的lncRNAs，其中31个在我们的样本中得到验证。与癌旁组织相比，大多数lncRNAs以及miR-200家族所有成员在癌组织中均呈现一致性下调表达。我们观察到至少一个miR-200家族成员与17个lncRNAs的表达存在显著相关性。特别值得注意的是，三个lncRNAs（MALAT1、OIP5-AS1和LINC00467）与miR-200家族所有成员的表达均显示出相关性。我们的结果在TCGA数据集的KIRC样本中至少得到部分验证。结论：我们的研究结果表明，RCC中miR-200家族的表达可能至少部分受到lncRNAs的调控。基于我们的样本队列分析，MALAT1、OIP5-AS1和LINC00467可能是影响RCC发生发展的重要潜在因子。
肿瘤(癌症)患者之家