Background: Effusion-based lymphoma (EBL) is a rare and aggressive large B-cell lymphoma. It presents as a body cavity effusion without a solid mass, lacks HHV-8 association, and typically expresses CD20. Objectives: To better understand the biology of this entity, we performed transcriptomic profiling of eight EBL cases. Methods: We analyzed the cases with the NanoString PanCancer Immune Profiling Panel and compared the results with publicly available datasets representing follicular lymphoma (FL), mantle cell lymphoma (MCL), and large B-cell lymphoma (LBCL) subtypes. Results: Unsupervised clustering and differential expression analysis revealed that EBL cases cluster transcriptionally with the LBCL group. Lymphoma-specific signaling pathway enrichment (SignatureDB) predominantly identified non-germinal center (activated B-cell-type) pathways. In addition, KEGG pathway analyses revealed enrichment in specific inflammatory and immune response pathways that are associated with B-cell lymphoma development in the setting of chronic inflammation, including those linked to Toll-like receptor and NF-κB signaling. Conclusions: These findings support a post-germinal center origin for EBL, which arises in a background of chronic inflammation and persistent antigen stimulation.
背景：积液性淋巴瘤（EBL）是一种罕见且侵袭性的大B细胞淋巴瘤。其表现为体腔积液而无实体肿块，不伴有HHV-8感染，通常表达CD20。目的：为深入理解该疾病的生物学特性，我们对八例EBL病例进行了转录组学分析。方法：采用NanoString PanCancer免疫分析芯片对病例进行检测，并将结果与公开的滤泡性淋巴瘤（FL）、套细胞淋巴瘤（MCL）及大B细胞淋巴瘤（LBCL）亚型数据集进行比较。结果：无监督聚类与差异表达分析显示，EBL病例在转录水平上与LBCL组聚类。淋巴瘤特异性信号通路富集分析（SignatureDB）主要识别出非生发中心（活化B细胞型）通路。此外，KEGG通路分析揭示了特定炎症与免疫应答通路的富集，这些通路与慢性炎症背景下B细胞淋巴瘤的发展相关，包括与Toll样受体及NF-κB信号传导相关的通路。结论：这些发现支持EBL起源于生发中心后阶段，并在慢性炎症与持续抗原刺激的背景下发生发展。
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