Background: miRNA profiling across different stages of laryngeal carcinogenesis explores dysregulated molecules relevant to engaged gene pathways and identifies markers for differential diagnosis and prognosis in early mucosal lesions of the larynx.Methods: Tissue samples were prospectively collected from 28 patients with hypertrophic vocal fold lesions: no dysplasia (ND), low-grade dysplasia (LGD), high-grade dysplasia (HGD), and invasive cancer (IC), as well as from 3 patients with vocal fold polyps. miRNA profiling of the samples was performed using microfluidic cards—TaqMan®Human MicroRNA Array A. A comparative analysis of ΔCt (dCt) miRNA expression levels was conducted between groups.Results: hsa-miR-216a-5p and hsa-miR-488-3p were selectively expressed in control tissues, while hsa-miR-105-5p and hsa-miR-516a-5p were exclusively detected in HGD and IC samples. Significant differences in miRNA expression were identified across 4, 16, 17, and 38 miRNA types between control and ND, LGD, HGD, and IC groups, respectively. hsa-miR-185-5p and hsa-miR-21-5p showed significantly altered expression between ND and LGD, HGD, and IC (p= 0.026, 0.001, 0.002; andp= 0.021, 0.002, 0.001, respectively). Twenty-five miRNAs were differentially expressed between LGD and both HGD and IC, while eleven miRNAs distinguished HGD from IC. Notably, hsa-miR-503-5p expression decreased progressively with increasing histological severity.Conclusions: Distinct miRNA expression profiles are associated with progressive stages of laryngeal mucosal lesions. Specific miRNAs may serve as valuable biomarkers for early detection, risk stratification, and prognosis in vocal fold carcinogenesis.
背景：通过对喉癌发生不同阶段的miRNA谱分析，可探索与相关基因通路失调有关的分子，并鉴定用于喉部早期黏膜病变鉴别诊断和预后的标志物。 方法：前瞻性收集28例声带肥厚性病变患者的组织样本，包括无异型增生（ND）、低度异型增生（LGD）、高度异型增生（HGD）和浸润癌（IC），以及3例声带息肉患者的样本。使用微流控芯片TaqMan®人类MicroRNA阵列A对样本进行miRNA谱分析。对各组间ΔCt（dCt）miRNA表达水平进行比较分析。 结果：hsa-miR-216a-5p和hsa-miR-488-3p在对照组组织中特异性表达，而hsa-miR-105-5p和hsa-miR-516a-5p仅在HGD和IC样本中检测到。对照组与ND、LGD、HGD和IC组之间分别有4、16、17和38种miRNA表达存在显著差异。hsa-miR-185-5p和hsa-miR-21-5p在ND与LGD、HGD和IC之间的表达显著改变（p值分别为0.026、0.001、0.002和0.021、0.002、0.001）。25种miRNA在LGD与HGD及IC之间存在差异表达，而11种miRNA可区分HGD与IC。值得注意的是，hsa-miR-503-5p的表达随组织学严重程度增加而逐渐降低。 结论：不同的miRNA表达谱与喉黏膜病变的进展阶段相关。特定miRNA可能作为声带癌变早期检测、风险分层和预后的有价值的生物标志物。
MiRNA Profiling in Premalignant Lesions and Early Glottic Cancer
肿瘤(癌症)患者之家