Background/Objectives: Electrochemotherapy (ECT) has been shown to be effective in treating colorectal liver metastases when combined with bleomycin (BLM). Based on this promising finding, we compared in this study the efficacy of BLM with oxaliplatin (OXP) and bevacizumab (BVZ) in ECT. Methods: WAG/Rij rats were randomized into three groups and underwent ECT with intravenous injection of BLM, OXP, or OXP with BVZ for eight days following hepatic tumor cell implantation. Ultrasound and photoacoustic imaging served to assess oxygen saturation (SO2) and hemoglobin concentration (HbT) of the developing tumors. Tissue samples were analyzed by histology and immunohistochemistry. Results: BLM treatment significantly reduced SO2(33.7%) and HbT (12.7%) levels compared to pretreatment values. In contrast, the OXP-treated groups exhibited only modest reductions in both parameters. BLM also induced a markedly higher necrosis rate (82.6%) compared to OXP and OXP/BVZ (11.0% and 26.3%). Conversely, OXP-treated tumors exhibited higher apoptosis rates. Furthermore, BLM treatment led to a decrease in tumor cell proliferation and a reduction in inflammatory response compared to the other treatments. Notably, BLM caused a 26.2% reduction in CD31-positive microvessels, which was significantly higher than that observed in the OXP group.Conclusions: BLM showed a more effective anti-tumor activity than OXP, suggesting its preferred use as chemotherapeutic agent in ECT.
背景/目的：电化学疗法联合博来霉素已被证实对结直肠癌肝转移具有良好疗效。基于这一前景，本研究比较了博来霉素与奥沙利铂及贝伐珠单抗在电化学疗法中的疗效差异。方法：将WAG/Rij大鼠随机分为三组，在肝肿瘤细胞植入后分别接受静脉注射博来霉素、奥沙利铂或奥沙利铂联合贝伐珠单抗的电化学治疗，持续八天。通过超声及光声成像技术监测肿瘤发展过程中的氧饱和度与血红蛋白浓度变化。对组织样本进行组织学及免疫组化分析。结果：与治疗前相比，博来霉素治疗组肿瘤氧饱和度显著降低33.7%，血红蛋白浓度下降12.7%；而奥沙利铂治疗组两项参数仅出现轻微下降。组织学分析显示，博来霉素组肿瘤坏死率高达82.6%，显著优于奥沙利铂组（11.0%）及奥沙利铂/贝伐珠单抗联合组（26.3%）。相反，奥沙利铂治疗组肿瘤细胞凋亡率更高。此外，博来霉素治疗能更有效抑制肿瘤细胞增殖并减轻炎症反应。值得注意的是，博来霉素组CD31阳性微血管密度降低26.2%，该效果显著优于奥沙利铂组。结论：博来霉素较奥沙利铂展现出更强的抗肿瘤活性，提示其作为电化学疗法化疗药物的优选地位。
肿瘤(癌症)患者之家