Background/Objectives: Flow cytometric analysis of cellular DNA content has prognostic importance in ovarian cancer and its measurement could contribute to clinical practice. The aim of this study was to compare serous cystadenoma and serous cystadenocarcinoma cases in terms of their flow cytometric analysis results.Methods: In total, 60 serous ovarian tumor cases (30 cases of ovarian serous cyst adenoma and 30 cases of ovarian serous cystadenocarcinoma) in paraffin blocks were extracted from hospital pathology archives for flow cytometric analysis. Cell cycle changes, aneuploidy ratio, and annexin V levels were compared.Results: The S phase cell ratio, proliferative index, aneuploidy cell ratio, and annexin V apoptotic index were significantly higher in the cancer group compared to the cystadenoma group. However, the G2/M phase cell ratio was found to be similar in both groups. When the annexin V apoptotic index cutoff value was selected as 27.65%, the sensitivity was calculated as 90.0% and the specificity as 93.3% for predicting serous ovarian carcinoma (AUC, 0.872;p< 0.001; 95% CI, 0.761–0.984).Conclusions: In serous cystadenocarcinoma cases, cell cycle activity was increased compared to serous cyst adenoma, but the similarity of G2/M activities suggested that serous cystadenocarcinoma may be a member of the “metaneoplasia” process. The detection of annexin V expression in ovarian tumors by flow cytometric analysis is a cheap, fast, and easy diagnostic method that can be used in the diagnosis of ovarian cancer. Annexin V expression is an effective biomarker with high sensitivity and specificity that could be used in the diagnosis of serous ovarian cancer.
背景/目的：细胞DNA含量的流式细胞术分析在卵巢癌中具有重要预后价值，其测量可为临床实践提供参考。本研究旨在比较浆液性囊腺瘤与浆液性囊腺癌病例的流式细胞术分析结果。 方法：从医院病理档案中提取石蜡包埋的60例浆液性卵巢肿瘤标本（30例卵巢浆液性囊腺瘤及30例卵巢浆液性囊腺癌）进行流式细胞术分析，比较细胞周期变化、非整倍体比例及膜联蛋白V水平。 结果：与囊腺瘤组相比，癌症组的S期细胞比例、增殖指数、非整倍体细胞比例及膜联蛋白V凋亡指数均显著升高，但两组G2/M期细胞比例相似。当膜联蛋白V凋亡指数截断值设定为27.65%时，其对浆液性卵巢癌的预测敏感度为90.0%，特异度为93.3%（AUC值为0.872；p<0.001；95%置信区间0.761-0.984）。 结论：浆液性囊腺癌病例的细胞周期活性较浆液性囊腺瘤增强，但G2/M期活性的相似性提示浆液性囊腺癌可能属于"化生性肿瘤"进程。通过流式细胞术检测卵巢肿瘤中膜联蛋白V表达是一种经济、快速且简便的诊断方法，可用于卵巢癌诊断。膜联蛋白V表达作为具有高敏感度与特异度的有效生物标志物，在浆液性卵巢癌诊断中具有应用价值。
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