Background/Objectives: Yes-associated protein (YAP) and transcriptional coactivator with PDZ-binding motif (TAZ) function as effectors in the Hippo pathway and have attracted attention due to their association with tumor formation. Glucose transporter (GLUT) proteins also contribute to the proliferation of cancer cells. In this study, we investigated the effect of YAP/TAZ on GLUT1 expression in endometrial carcinoma, as well as the clinical relevance and prognostic value of YAP/TAZ.Methods: The effects of YAP and TAZ knockdown and YAP overexpression on GLUT1 expression in human endometrial carcinoma-derived HHUA and Ishikawa cells were evaluated using RT-qPCR. In addition, we performed immunohistochemical expression of 100 tissue samples of diagnosed endometrial carcinoma. Based on staining intensity and the percentage of positively stained tumor cells, the immunoreactivity score was calculated, which ranged from 0 to 12.Results: YAP/TAZ were identified as important factors in the regulation of GLUT1 expression in HHUA and Ishikawa cells. In addition, a significant correlation (progression-free survivalp< 0.05) was observed between TAZ and GLUT1 expression in tissues from endometrial carcinoma patients, and nuclear expression of TAZ was associated with poor prognosis (p< 0.05).Conclusions: YAP/TAZ promote tumor growth via GLUT1. Therapeutic targeting of YAP/TAZ could therefore be useful in the development of future treatments.
背景/目的：Yes相关蛋白（YAP）和具有PDZ结合基序的转录共激活因子（TAZ）作为Hippo通路中的效应分子，因其与肿瘤形成的关联而备受关注。葡萄糖转运蛋白（GLUT）同样参与癌细胞的增殖过程。本研究旨在探讨YAP/TAZ对子宫内膜癌中GLUT1表达的影响，并分析YAP/TAZ的临床相关性及预后价值。 方法：通过RT-qPCR技术评估敲低YAP/TAZ及过表达YAP对人子宫内膜癌细胞系HHUA和Ishikawa中GLUT1表达的影响。同时，对100例确诊子宫内膜癌组织样本进行免疫组化染色分析。根据染色强度与阳性染色肿瘤细胞百分比计算免疫反应评分（评分范围0-12分）。 结果：研究发现YAP/TAZ是调控HHUA和Ishikawa细胞中GLUT1表达的关键因子。在子宫内膜癌患者组织中，TAZ与GLUT1表达呈显著正相关（无进展生存期p<0.05），且TAZ核表达与不良预后显著相关（p<0.05）。 结论：YAP/TAZ通过GLUT1途径促进肿瘤生长。因此，靶向YAP/TAZ的治疗策略可能为未来治疗方案开发提供新方向。
YAP/TAZ Promote GLUT1 Expression and Are Associated with Prognosis in Endometrial Cancer
肿瘤(癌症)患者之家