Background: Currently, much attention is focused on the interactions between the leukemic and psoriatic cells showing immunosuppressive activity within the microenvironment. Methods: Our study assessed a collective mRNA expression pattern of crucial immuno-regulatory genes:BTLA,CD160,SPN,TIM-3,VISTA,TIGIT, by qRT-PCR, and performed a comparison in two different diseases, chronic lymphocytic leukemia (CLL) and psoriasis (Ps), referring to clinical characteristics. Results: In Ps, all the studied gene expressions, exceptTIM-3, were higher than in HVs and all the studied gene expressions, exceptVISTA, were lower than in CLL. However, the expression ofTIM-3, a checkpoint inhibitor, was higher in 0 stage of CLL and was lower in advanced stages of the disease, suggesting its possible diagnostic value. Expression ofVISTAwas higher in Ps than in HVs, as well as in CLL. It is noteworthy thatBTLA,CD160andSPNwere overexpressed in CLL and Ps compared to HVs, suggesting its involvement in immune suppression in both diseases. Conclusions: Significant correlations between gene expressions ofSPNandBTLA,SPNandTIGIT,CD160andTIM-3, were observed, indicating a potential shared regulatory mechanism for immune responses which suggests their bidirectional regulatory role on the functioning of immune system cells, depending on the context of inflammatory or neoplastic conditions.
背景：目前，研究焦点集中于白血病细胞与银屑病细胞在微环境中表现出的免疫抑制活性及其相互作用。方法：本研究通过定量逆转录聚合酶链反应（qRT-PCR）评估了关键免疫调节基因（BTLA、CD160、SPN、TIM-3、VISTA、TIGIT）的总体mRNA表达模式，并参照临床特征对慢性淋巴细胞白血病（CLL）和银屑病（Ps）两种不同疾病进行了比较分析。结果：在银屑病患者中，除TIM-3外所有研究基因的表达均高于健康志愿者；除VISTA外所有基因的表达均低于CLL患者。值得注意的是，免疫检查点抑制剂TIM-3在CLL 0期的表达较高，在疾病进展期表达降低，提示其可能具有诊断价值。VISTA在银屑病和CLL中的表达均高于健康对照组。与健康志愿者相比，BTLA、CD160和SPN在CLL和银屑病中均呈现过表达，表明这些基因可能参与两种疾病的免疫抑制过程。结论：研究观察到SPN与BTLA、SPN与TIGIT、CD160与TIM-3基因表达之间存在显著相关性，提示免疫反应可能存在共同的调控机制。这表明这些基因根据炎症或肿瘤性疾病的具体环境，对免疫系统细胞功能具有双向调节作用。
肿瘤(癌症)患者之家