Flow cytometry use has significantly increased in clinical laboratories and has significantly helped improve the diagnosis of leukemias, lymphomas, and follow-up of minimal residual disease. Mastering this technique enables the performance of multiparametric single-cell analysis and increases the odds of identifying abnormal populations. As in many fields, there is a need to improve the quality of the data generated for accuracy, reproducibility, and trueness. The implementation of solutions reducing variability is achievable and needed, as the flow cytometry workflow involves many manual steps and items susceptible to operator bias and human error. Standardization of flow cytometry assays is sought and already implemented in many clinical hematology laboratories. However, the clinical community would highly benefit from further efforts in that direction to increase the comparability of findings across laboratories. This review covers the strengths and weaknesses of flow cytometry and focuses on the standardization approaches developed, including recent advances in the field.
流式细胞术在临床实验室中的应用显著增加，并极大地促进了白血病、淋巴瘤的诊断以及微小残留病的监测。掌握该技术能够实现多参数单细胞分析，提高异常细胞群的检出率。与许多领域一样，为确保数据的准确性、可重复性和真实性，需要提升生成数据的质量。由于流式细胞术工作流程涉及大量人工操作环节，易受操作者偏差和人为误差影响，因此实施降低变异性的解决方案是可行且必要的。流式细胞术检测的标准化已成为临床血液学实验室的普遍追求，并在许多机构中得以实施。然而，临床领域仍需在该方向进一步努力，以增强不同实验室间检测结果的可比性。本文综述了流式细胞术的优势与局限，重点探讨了已建立的标准化方案，包括该领域的最新进展。
An Update on Flow Cytometry Analysis of Hematological Malignancies: Focus on Standardization
肿瘤(癌症)患者之家