Background/Objectives: Pancreatic cancer is the fourth leading cause of deaths related to cancer. It is a highly aggressive malignancy and often metastasizes quickly to other parts of the body and organs. The most effective cure is surgical resection, which also is limited by tumor identification and clear tumor margin visualization. Previously, we used MUC4 antibodies labeled with IRDye800CW (anti-MUC4-IR800) to target primary human pancreatic cancer in orthotopic cell line mouse models. Methods: In the present study, we established a pancreatic cancer liver metastasis mouse model by implanting a tumor fragment in the liver and a peritoneal carcinomatosis mouse model by injecting pancreatic cancer cells interperitoneally. Once the tumors were established, anti-MUC4-IR800 was administered to the mice by tail vein injection. Laparotomy was performed and tumors were imaged under white-light and near-infrared (NIR) fluorescence with the Pearl Small Animal Imaging System. Results: NIR imaging after 72 h shows the bright targeting of pancreatic cancer metastasis in both mouse models with high tumor-to-background ratios. Conclusions: Anti-MUC4-IR800 was able to successfully target and brightly label metastatic pancreatic cancer as small as 1 mm. Future clinical applications of the results of the present study are discussed.
背景/目的：胰腺癌是癌症相关死亡的第四大主要原因。这是一种高度侵袭性的恶性肿瘤，常迅速转移至身体其他部位和器官。最有效的治疗方法是手术切除，但该方法也受限于肿瘤识别和清晰肿瘤边缘的可视化。先前，我们使用标记有IRDye800CW的MUC4抗体（抗MUC4-IR800）在原位细胞系小鼠模型中靶向原发性人类胰腺癌。方法：在本研究中，我们通过在肝脏植入肿瘤片段建立了胰腺癌肝转移小鼠模型，并通过腹腔注射胰腺癌细胞建立了腹膜癌病小鼠模型。肿瘤形成后，通过尾静脉注射向小鼠给予抗MUC4-IR800。进行剖腹手术，并使用Pearl小动物成像系统在白光和近红外荧光下对肿瘤进行成像。结果：72小时后的近红外成像显示，两种小鼠模型中的胰腺癌转移灶均呈现明亮的靶向信号，且肿瘤与背景比值较高。结论：抗MUC4-IR800能够成功靶向并明亮标记小至1毫米的转移性胰腺癌。本文讨论了本研究结果未来的临床应用前景。
肿瘤(癌症)患者之家