文章：

五个细胞基因作为宫颈腺癌分子标志物的候选者

Five Cellular Genes as Candidates for Cervical Adenocarcinoma Molecular Markers

原文发布日期：3 May 2025

DOI: 10.3390/cancers17091558

类型: Article

开放获取: 是

英文摘要：

Background/Aim: Cervical adenocarcinoma associated with Human Papillomavirus (HPV) infection represents 85–90% of all adenocarcinomas that have poor prognostic factors and is an important health public concern. Currently, cervical adenocarcinoma molecular markers are scarce. This study searched databases and the literature regarding candidate genes to find these molecular markers, which were experimentally evaluated in fresh cervical samples. Materials and Methods: Bioinformatic analysis of 161 transcriptomic libraries of cervical tissues with or without lesions from the NCBI database was performed using the Partek Genomics Suite 6.6v software. The selected genes with apvalue of >0.05, and 1.5-fold change were considered. A search of molecular marker candidates of cervical lesions that were already published in the literature was performed. To validate the selected genes, total RNA from fresh cervical adenocarcinoma and cervical normal tissues were subjected to RT-PCR experiments; HPV detection was also performed. Results: Initially, twenty-five genes were identified using bioinformatic analysis, and their expression was evaluated. The results showed that theHOXC6,HOXC8,RARβ,ELAVL2,URG4,CISD2,CA9,BCL2,Survivin,MACC1,CDKN2A, and HPVE6/E7genes were found to be differentially expressed in CC. Among these,RARβ,MACC1,BCL2,HOXC8, and E6/E7/HPV exhibited higher statistical significance for CC samples. Conclusions: This five-gene panel could serve as a novel molecular tool for HPV-associated cervical adenocarcinoma detection.

摘要翻译： 

背景/目的：与人乳头瘤病毒（HPV）感染相关的宫颈腺癌占所有具有不良预后因素的腺癌的85-90%，是公共卫生领域的重要关注点。目前，宫颈腺癌的分子标志物较为稀缺。本研究通过检索数据库及相关文献，筛选候选基因以寻找此类分子标志物，并在新鲜宫颈样本中进行了实验验证。材料与方法：使用Partek Genomics Suite 6.6v软件对NCBI数据库中161份有或无病变的宫颈组织转录组文库进行生物信息学分析，筛选标准为p值>0.05且表达差异倍数>1.5的基因。同时系统检索了已发表的宫颈病变分子标志物相关文献。为验证所选基因，对新鲜宫颈腺癌组织及正常宫颈组织的总RNA进行RT-PCR实验，并同步完成HPV检测。结果：通过生物信息学分析初步鉴定出25个基因并进行表达评估。结果显示，HOXC6、HOXC8、RARβ、ELAVL2、URG4、CISD2、CA9、BCL2、Survivin、MACC1、CDKN2A及HPV E6/E7基因在宫颈癌中呈现差异表达。其中，RARβ、MACC1、BCL2、HOXC8及E6/E7/HPV在宫颈癌样本中表现出更高的统计学显著性。结论：该五基因组合可作为HPV相关宫颈腺癌检测的新型分子工具。

原文链接：

Five Cellular Genes as Candidates for Cervical Adenocarcinoma Molecular Markers