文章：

拷贝数变异与PRAME免疫组织化学初步研究在疑难黑色素细胞病变中的比较研究

A Comparative Study Between Copy Number Alterations and PRAME Immunohistochemical Pilot Study in Challenging Melanocytic Lesions

原文发布日期：4 April 2025

DOI: 10.3390/cancers17071218

类型: Article

开放获取: 是

英文摘要：

Introduction: Diagnostic uncertainty for ambiguous lesions that fall on the spectrum between nevi and melanoma remains a significant challenge and can have consequences for patient management. Methods: This study aimed to compare the diagnostic utility of preferentially expressed antigen in melanoma (PRAME) immunohistochemistry to molecular testing (FISH and SNP array) in 34 diagnostically challenging melanocytic lesions and 9 non-diagnostically challenging melanomas. Results: We conclude that while PRAME immunohistochemistry demonstrates high specificity (96.2%) in diagnostically challenging melanocytic lesions, its low sensitivity (12.5%) suggests that it should not replace histopathological evaluation in rendering the final diagnosis. Conclusions: These findings suggest that PRAME may serve as a useful adjunct in the diagnostic workup, particularly due to its high negative predictive value, but should be used in conjunction with other established diagnostic modalities.

摘要翻译： 

引言：对于介于痣与黑色素瘤之间的不明确病变，诊断不确定性仍是一个重大挑战，并可能影响患者的临床管理。方法：本研究旨在比较黑色素瘤优先表达抗原（PRAME）免疫组化检测与分子检测（荧光原位杂交和单核苷酸多态性阵列）在34例诊断困难的黑色素细胞病变和9例诊断明确的黑色素瘤中的诊断效用。结果：我们发现，尽管PRAME免疫组化在诊断困难的黑色素细胞病变中表现出高特异性（96.2%），但其低敏感性（12.5%）表明它不应取代组织病理学评估作为最终诊断依据。结论：这些发现提示PRAME可作为诊断过程中的有效辅助手段，特别是因其较高的阴性预测价值，但应与其他成熟的诊断方法结合使用。

原文链接：

A Comparative Study Between Copy Number Alterations and PRAME Immunohistochemical Pilot Study in Challenging Melanocytic Lesions