Background/Objectives:Colorectal cancer (CRC) is a major global health concern with rising incidence and mortality. MicroRNAs (miRNAs) play key roles in tumor progression, invasion, and metastasis. This study aimed to analyze the expression of miR-101-3p, miR-106a-5p, and miR-326 in CRC patients and assess their association with tumor grading and tissue invasion.Methods:A total of 40 CRC patients were included in the study. miRNA expression levels were measured using quantitative reverse transcription PCR (qRT-PCR). Statistical analyses, including regression models, were performed to evaluate correlations between miRNA expression, tumor grade, and invasion patterns.Results:MiR-101-3p was significantly downregulated in advanced tumors, while miR-106a-5p expression peaked in Grade 2 CRC. MiR-326 expression was associated with organ metastasis. Regression models confirmed complex interaction patterns among these miRNAs, suggesting their potential role in CRC progression.Conclusions:These findings indicate that miR-101-3p, miR-106a-5p, and miR-326 are associated with CRC severity and invasion. Their expression patterns suggest potential utility as biomarkers for early detection and targeted therapies. Further studies with larger cohorts are needed to validate these results and explore their clinical applications.
**背景/目的：** 结直肠癌是全球重大健康问题，其发病率和死亡率持续上升。微小RNA在肿瘤进展、侵袭和转移过程中发挥关键作用。本研究旨在分析结直肠癌患者中miR-101-3p、miR-106a-5p和miR-326的表达水平，并评估其与肿瘤分级及组织侵袭性的关联。 **方法：** 研究共纳入40例结直肠癌患者。采用定量逆转录聚合酶链反应检测miRNA表达水平。通过回归模型等统计方法分析miRNA表达与肿瘤分级、侵袭模式之间的相关性。 **结果：** miR-101-3p在晚期肿瘤中显著下调，而miR-106a-5p在2级结直肠癌中表达达到峰值。miR-326表达与器官转移相关。回归模型证实这些miRNA之间存在复杂的相互作用模式，提示其在结直肠癌进展中的潜在作用。 **结论：** 本研究表明miR-101-3p、miR-106a-5p和miR-326与结直肠癌严重程度及侵袭性相关。其表达模式提示这些分子可能作为早期诊断标志物和靶向治疗的潜在靶点。未来需要更大规模队列研究验证结果并探索其临床应用价值。
The Impact of miRNA Expression on Colon Cancer Severity, Invasiveness, and Localization
肿瘤(癌症)患者之家