Background: Ionizing radiation (IR) exposure poses a significant health risk due to its widespread use in medical diagnostics and therapeutic applications, necessitating rapid and effective biomarkers for assessment. Objective: The aim of this study is to identify the serum proteomic signature of IR exposure in patients undergoing radiotherapy (RT). Methods: Blood samples were obtained from eighteen patients with head and neck cancer (HNC) and five patients with rectal cancer before and immediately after they underwent curative intensity-modulated radiotherapy (IMRT). The comprehensive serum proteome was analyzed in individual samples using nanoHPLC-MS/MS. Results: Forty radiation-modulated proteins (RMPs), 24 upregulated and 16 downregulated, with a fold change ≥1.5 andp-value < 0.05 were identified. About 40% of the RMPs are involved in acute phase response, DNA repair, and inflammation; the key RMPs wereADCY1,HGF,MCEMP1,CHD4,RECQL5,MSH6, andZNF224.Conclusions: This study identifies a panel of serum proteins that may reflect the radiation response, providing a valuable molecular fingerprint of IR exposure and paving the way for the development of sensitive and specific biomarkers for early detection and clinical management of IR-related injuries.
背景：电离辐射（IR）在医学诊断和治疗中广泛应用，其暴露构成显著健康风险，因此需要快速有效的生物标志物进行评估。目的：本研究旨在识别接受放射治疗（RT）患者血清中电离辐射暴露的蛋白质组学特征。方法：收集18例头颈癌（HNC）患者和5例直肠癌患者在根治性调强放疗（IMRT）前及治疗结束后的血液样本，采用纳升级高效液相色谱-串联质谱技术对个体样本进行全血清蛋白质组分析。结果：共鉴定出40种辐射调控蛋白（RMPs），其中24种上调、16种下调（变化倍数≥1.5且p值<0.05）。约40%的RMPs参与急性期反应、DNA修复和炎症过程，关键蛋白包括ADCY1、HGF、MCEMP1、CHD4、RECQL5、MSH6和ZNF224。结论：本研究筛选出一组可能反映辐射反应的血清蛋白，为电离辐射暴露提供了有价值的分子指纹图谱，为开发灵敏特异的生物标志物以早期检测和临床管理辐射相关损伤奠定了基础。
Serum-Based Proteomic Approach to Identify Clinical Biomarkers of Radiation Exposure
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