Background: Hepatocellular carcinoma (HCC) and intrahepatic cholangiocarcinoma (ICC) are two distinct types of primary liver cancer (PLC) characterized by considerable extents of cellular and molecular heterogeneities. We recently developed a web-based cell atlas called LiverSCA that possesses a user-friendly interface and comprehensive functionalities. It facilitates the exploration of gene expression patterns, cellular compositions, and intercellular communication within the microenvironments of liver and PLC tumors. Methods: To further enhance the documentation of data pinpointing different phenotypes/subtypes of liver and PLC, we extended the catalog of LiverSCA with additional datasets, e.g., ICC and metabolic dysfunction-associated steatotic liver disease/steatosis (MASLD/MASH). Results: The current enhanced version of the LiverSCA cell atlas encompasses six phenotypes (normal, HBV-HCC, HCV-HCC, non-viral HCC, ICC, and MASH), 63 patients, and over 248,000 cells. Furthermore, we have incorporated comparative visualization methods that allow users to simultaneously examine and compare gene expression levels between two different phenotypes. Conclusions: We are committed to the continuous development of LiverSCA and envision that it will serve as a valuable resource to support researchers in convenient investigations into the cellular and molecular landscapes of liver and PLC.
背景：肝细胞癌（HCC）和肝内胆管癌（ICC）是两种不同类型的原发性肝癌（PLC），具有显著的细胞和分子异质性。我们近期开发了一个名为LiverSCA的在线细胞图谱，该平台界面友好、功能全面，能够帮助研究者探索肝脏及PLC肿瘤微环境中的基因表达模式、细胞组成及细胞间通讯机制。 方法：为进一步完善肝脏及PLC不同表型/亚型的数据记录，我们在LiverSCA原有数据库基础上扩展了数据集，新增了ICC及代谢功能障碍相关脂肪性肝病/脂肪性肝炎（MASLD/MASH）等数据。 结果：当前升级版的LiverSCA细胞图谱涵盖六种表型（正常组织、HBV-HCC、HCV-HCC、非病毒性HCC、ICC及MASH），包含63例患者样本及超过24.8万个细胞数据。此外，我们整合了对比可视化功能，支持用户同时对两种不同表型的基因表达水平进行观察与比较。 结论：我们将持续完善LiverSCA平台的建设，期待该资源库能为研究者探索肝脏及PLC的细胞与分子特征提供便捷有效的支持。
肿瘤(癌症)患者之家