文章：

急性白血病谱系不明：流式细胞术的诊断与评估

Acute Leukemia of Ambiguous Lineage: Diagnosis and Evaluation by Flow Cytometry

原文发布日期：3 March 2025

DOI: 10.3390/cancers17050871

类型: Article

开放获取: 是

英文摘要：

Acute leukemia of ambiguous lineage (ALAL) includes mixed-phenotype acute leukemia (MPAL), which exhibits immunophenotypic evidence of differentiation along more than one cell lineage, and acute undifferentiated leukemia (AUL), which lacks sufficient immunophenotypic differentiation along any cell lineage. This review provides an overview of ALAL, emphasizing the central role of flow cytometric analysis in its diagnostic workflow. It primarily focuses on MPAL, addressing updated classification and diagnostic criteria by the WHO-HEM5 and the ICC, including both genetically defined and phenotypically defined MPAL. The article provides a detailed review of the MPAL lineage assignment criteria with an illustrative description of a series of MPAL cases. Future studies are needed to reconcile the different criteria used in these two classifications. Continuously expanded molecular studies are expected to provide a genomic and lineage-associated framework for the classification of ALAL with clinical relevance in the diagnosis and therapy selection.

摘要翻译： 

谱系不明急性白血病（ALAL）包括混合表型急性白血病（MPAL）和急性未分化白血病（AUL）。MPAL表现为沿多个细胞谱系分化的免疫表型证据，而AUL则缺乏沿任何细胞谱系的充分免疫表型分化。本综述概述了ALAL，重点强调了流式细胞术分析在其诊断流程中的核心作用。文章主要聚焦于MPAL，探讨了WHO-HEM5和ICC更新的分类与诊断标准，涵盖遗传学定义和表型定义的MPAL。文中详细回顾了MPAL谱系判定标准，并通过一系列MPAL病例进行说明性描述。未来研究需协调这两种分类体系中的不同标准。不断扩展的分子研究有望为ALAL的分类提供基因组学和谱系关联框架，从而在诊断和治疗选择中具有临床相关性。

原文链接：

Acute Leukemia of Ambiguous Lineage: Diagnosis and Evaluation by Flow Cytometry