文章：

流式细胞术在B细胞非霍奇金淋巴瘤与霍奇金淋巴瘤中的应用

Flow Cytometry for B-Cell Non-Hodgkin and Hodgkin Lymphomas

原文发布日期：26 February 2025

DOI: 10.3390/cancers17050814

类型: Article

开放获取: 是

英文摘要：

Multi-parametric flow cytometry is a powerful diagnostic tool that permits rapid assessment of cellular antigen expression to quickly provide immunophenotypic information suitable for disease classification. This chapter describes the classification of B-cell non-Hodgkin lymphoma (B-NHL) by flow cytometry suitable for the clinical and research environment. In addition to describing the immunophenotypic patterns of the most common B-NHL (including examples of common B-NHL), the effect of anti-CD19, -CD20, and -CD38 therapies on the evaluation of flow cytometric data is also discussed. Over the last 15 years, our laboratory has developed flow cytometry combinations that can immunophenotype classic Hodgkin lymphoma (CHL), nodular lymphocyte predominant Hodgkin lymphoma (NLPHL), and T-cell/histocyte-rich large B-cell lymphoma (THRLBCL) and the use of these assays will be presented. The CHL assay combination is also particularly well suited to immunophenotype primary mediastinal large B-cell lymphoma (PMLBCL) and our experience immunophenotyping PMLBCL by flow cytometry will be discussed. Finally, an approach to the evaluation of the reactive infiltrate of CHL, NLPHL, and THRLBCL that can provide diagnostic information will also be provided.

摘要翻译： 

多参数流式细胞术是一种强大的诊断工具，能够通过快速评估细胞抗原表达，及时提供适用于疾病分类的免疫表型信息。本章阐述了适用于临床与研究环境的B细胞非霍奇金淋巴瘤（B-NHL）流式细胞术分类方法。除描述最常见B-NHL的免疫表型特征（包括典型病例示例）外，还探讨了抗CD19、抗CD20及抗CD38治疗对流式细胞术数据判读的影响。过去十五年间，本实验室研发了可用于经典霍奇金淋巴瘤（CHL）、结节性淋巴细胞为主型霍奇金淋巴瘤（NLPHL）及富含T细胞/组织细胞的大B细胞淋巴瘤（THRLBCL）免疫表型分析的流式细胞术组合方案，本章将系统介绍这些检测方法的应用。其中CHL检测方案尤其适用于原发性纵隔大B细胞淋巴瘤（PMLBCL）的免疫表型分析，我们将详细探讨运用流式细胞术进行PMLBCL免疫表型检测的实践经验。最后，本章还将提出一套针对CHL、NLPHL及THRLBCL反应性浸润细胞的评估方案，该方案可为临床诊断提供重要参考信息。

原文链接：

Flow Cytometry for B-Cell Non-Hodgkin and Hodgkin Lymphomas