文章：

荧光引导下GL261红荧光素酶与TRP-mCherry荧光素酶小鼠胶质母细胞瘤肿瘤切除术

Fluorescence-Guided Resection of GL261 Red-FLuc and TRP-mCherry-FLuc Mouse Glioblastoma Tumors

原文发布日期：21 February 2025

DOI: 10.3390/cancers17050734

类型: Article

开放获取: 是

英文摘要：

Background: Most preclinical studies on glioblastoma (GBM) fail to provide translational utility in the clinic. Fluorescence-guided surgery using 5-aminolevulinic acid (5-ALA) improves tumor resection, disease prognosis, and, thus, patient outcomes. Given the critical role of surgery in managing recurrent GBM, it is essential to incorporate surgical elements into preclinical models to accurately reflect clinical scenarios and enhance translational success. However, existing protocols for 5-ALA-guided resection in preclinical models are limited and often lack clinical relevance.Methods: To address this gap, we developed a novel protocol for the 5-ALA-guided resection in two mouse GBM models: TRP-mCherry-FLuc and GL261 Red-FLuc.Results: The resection of TRP-mCherry-FLuc tumors significantly extended survival and mitigated weight loss compared to controls. Similarly, GL261 Red-FLuc tumor resection increased survival, reduced body weight loss, and slowed tumor progression.Conclusions: This study presents a clinically relevant protocol for 5-ALA-guided resection in preclinical GBM models, providing a platform for future research to integrate adjuvant therapies and enhance their potential translation into clinical practice.

摘要翻译： 

背景：大多数关于胶质母细胞瘤（GBM）的临床前研究未能转化为临床应用。使用5-氨基乙酰丙酸（5-ALA）进行荧光引导手术可改善肿瘤切除效果、疾病预后，从而提高患者治疗效果。鉴于手术在复发性GBM治疗中的关键作用，将手术要素纳入临床前模型以准确反映临床场景并提升转化成功率至关重要。然而，现有临床前模型中5-ALA引导切除的方案有限，且往往缺乏临床相关性。 方法：为填补这一空白，我们在两种小鼠GBM模型（TRP-mCherry-FLuc和GL261 Red-FLuc）中开发了一种新型5-ALA引导切除方案。 结果：与对照组相比，TRP-mCherry-FLuc肿瘤的切除显著延长了生存期并减轻了体重下降。同样，GL261 Red-FLuc肿瘤切除也提高了生存率，减少了体重下降，并延缓了肿瘤进展。 结论：本研究提出了一种具有临床相关性的临床前GBM模型5-ALA引导切除方案，为未来研究整合辅助疗法并促进其向临床实践转化提供了平台。

原文链接：

Fluorescence-Guided Resection of GL261 Red-FLuc and TRP-mCherry-FLuc Mouse Glioblastoma Tumors