文章：

口腔微生物组在口腔恶性肿瘤发展中的参与作用

Involvement of Oral Microbiome in the Development of Oral Malignancy

原文发布日期：13 February 2025

DOI: 10.3390/cancers17040632

类型: Article

开放获取: 是

英文摘要：

Objective: This study aimed to identify periodontal pathogens involved in the onset and progression of OSCC. Methods: Saliva samples were collected from 112 patients without oral mucosal diseases (OMDs) as controls; 36 patients with oral potentially malignant disorders (OPMDs); and 104 patients with OSCC. Periodontal examinations were performed on all patients. Endpoint PCR was performed for seven species of oral pathogens. The 16S rRNA analysis was performed using 20 DNA samples from each group. Results: Periodontitis tended to worsen in the OMDs group compared to the control group. The number of oral bacteria was significantly higher in the OSCC group than in the other groups. The detection rates ofP. gingivalisandAggregatibacter actinomycetemcomitans(A. actinomycetemcomitans) were significantly higher in the OSCC group than those in the control group. From 16S rRNA analysis, the relative abundance ofPrevotella buccaeandintermediawas significantly higher in OSCC than in the control. Moreover, LPS derived fromP. gingivaliscontributes to the early development of oral epithelial precancerous lesions and carcinomas in mice. Conclusions: Specific periodontal pathogens are present in the oral cavities of patients with OPMDs and OSCC, and changes in the bacterial flora due to their presence may contribute to the onset and progression of OMDs.

摘要翻译： 

目的：本研究旨在明确与口腔鳞状细胞癌（OSCC）发生及进展相关的牙周致病菌。方法：收集112例无口腔黏膜疾病（OMDs）患者作为对照组的唾液样本；36例口腔潜在恶性疾病（OPMDs）患者样本；以及104例OSCC患者样本。对所有患者进行牙周检查。采用终点PCR技术检测七种口腔致病菌。每组选取20份DNA样本进行16S rRNA分析。结果：与对照组相比，OMDs组患者的牙周炎病情呈加重趋势。OSCC组患者口腔细菌数量显著高于其他组。OSCC组中牙龈卟啉单胞菌和伴放线聚集杆菌的检出率显著高于对照组。16S rRNA分析显示，OSCC组中颊普雷沃菌和中间普雷沃菌的相对丰度显著高于对照组。此外，动物实验表明牙龈卟啉单胞菌来源的脂多糖可促进小鼠口腔上皮癌前病变及癌变的早期发展。结论：OPMDs和OSCC患者口腔中存在特异性牙周致病菌，这些菌群变化可能参与OMDs的发生与进展过程。

原文链接：

Involvement of Oral Microbiome in the Development of Oral Malignancy