Introduction.In medullary thyroid carcinoma (MTC), calcitonin levels and TNM staging are established prognostic factors. However, the diagnostic accuracy of cytology in MTC is poor. In this context, microRNAs (miRNAs) may be useful as valuable biomarkers, although evidence in MTC remains scarce. The aim of this study was to determine the expression of several miRNAs in patients with MTC and to investigate their association with clinical aggressiveness.Methods.Twenty-five patients undergoing surgery for MTC were included. miRNA expression profiles (miR-183-5p, miR-375-5p, miR-144-3p, miR-34a-5p, and miR-200c-3p) were analyzed in both histological samples of MTC and paired healthy thyroid tissue. cDNA synthesis was performed using the miRCURY LNA RT Kit (Exiqon Ref. 339340 Qiagen, Hilden, Germany). Gene expression was studied by real-time PCR using the QuantStudio 5 kit (Applied Biosystem). Variables included epidemiological, clinical, and follow-up variables. Statistical analysis: SPSS v.29. Kruskal–Wallis, Mann–Whitney U, and Spearman’s ρ tests were used.Results.Sixty percent (n= 15) were female and 56% (n= 14) had familial CMT. The mean age at diagnosis was 41.8 years. The median expression of miR-200c-3p, miR-144-3p, miR-34a-5p, miR183-5p, and miR-375-5p was 1.02, 0.923, 1.002, 7.666, and 2.226, respectively. Significant differences in expression were found for miR183-5p and 375-5p (p= 0.001) compared to paired healthy thyroid tissue. Patients with a higher disease-free survival (DFS) showed higher expression of 200c-3p (ρ = 0.456,p= 0.022).Conclusions.The expression of miRNAs 183-5p and 375-5p may help us to diagnose MTC, and a lower expression of miRNA 200C-3p may indicate a higher clinical aggressiveness.
引言。在甲状腺髓样癌（MTC）中，降钙素水平和TNM分期是公认的预后因素。然而，细胞学检查在MTC中的诊断准确性较差。在此背景下，微小RNA（miRNA）可能作为有价值的生物标志物发挥作用，尽管在MTC中的相关证据仍然有限。本研究旨在测定MTC患者中几种miRNA的表达水平，并探讨其与临床侵袭性的关联。 方法。研究纳入了25例接受MTC手术的患者。在MTC组织学样本及配对的健康甲状腺组织中分析了miRNA表达谱（miR-183-5p、miR-375-5p、miR-144-3p、miR-34a-5p和miR-200c-3p）。使用miRCURY LNA RT试剂盒（Exiqon，货号339340，Qiagen，德国希尔登）进行cDNA合成。采用QuantStudio 5试剂盒（Applied Biosystem）通过实时荧光定量PCR研究基因表达。变量包括流行病学、临床及随访变量。统计分析使用SPSS v.29软件，采用Kruskal-Wallis检验、Mann-Whitney U检验和Spearman ρ检验。 结果。60%（n=15）的患者为女性，56%（n=14）患有家族性MTC。诊断时的平均年龄为41.8岁。miR-200c-3p、miR-144-3p、miR-34a-5p、miR-183-5p和miR-375-5p的中位表达水平分别为1.02、0.923、1.002、7.666和2.226。与配对的健康甲状腺组织相比，miR-183-5p和miR-375-5p的表达存在显著差异（p=0.001）。无病生存期（DFS）较高的患者表现出更高的miR-200c-3p表达水平（ρ=0.456，p=0.022）。 结论。miR-183-5p和miR-375-5p的表达可能有助于MTC的诊断，而miR-200c-3p的低表达可能提示更高的临床侵袭性。
Could microRNA Analysis Help in the Management of Medullary Thyroid Cancer?
肿瘤(癌症)患者之家