Background and Objective: Access to high-quality patient-derived brain tumor tissues is instrumental for translational neuro-oncology research. Glioblastoma tumor material resected by ultrasonic aspiration (UA) during surgery offers an abundant source of material; however, it is generally not used for research experiments. We hypothesize that UA-derived tumor tissue represents a source of tissue that accurately reflects the immune infiltrates of glioblastomas.Methods: In this study, we have utilized UA-derived tissue and performed a head-to-head comparison with paired resection tissue from the vital tumor core of the same patient. A combination of 16 fluorochrome-conjugated antibodies was designed to identify tumor-infiltrating T, B, and NK lymphocytes and characterize the TILs by spectral flow cytometry. Furthermore, a 5-plex panel was designed to spatially characterize the T cells, macrophages, and tumor cells on the paired UA and resection tissues.Results: UA-obtained cells exhibited a comparable yield and viability, as well as an abundance of tumor-infiltrating T, B, and NK lymphocytes compared to resection sample-derived cells. Importantly, we observed that there is a high concordance with respect to expression intensities of immune checkpoints by T cells in both types of tissue samples.Conclusions: These findings underscore the feasibility and reliability of utilizing the immune infiltrates from ultrasonic aspiration-acquired glioblastoma tissue.
背景与目的：获取高质量的患者源性脑肿瘤组织对转化神经肿瘤学研究至关重要。手术中通过超声吸引术（UA）切除的胶质母细胞瘤组织提供了丰富的材料来源，但通常未用于研究实验。我们假设UA来源的肿瘤组织能准确反映胶质母细胞瘤免疫浸润情况。方法：本研究利用UA来源的组织，并与同一患者肿瘤核心区的配对切除组织进行直接比较。通过设计16色荧光标记抗体组合，利用光谱流式细胞术鉴定肿瘤浸润性T细胞、B细胞和NK淋巴细胞，并表征肿瘤浸润淋巴细胞的特征。此外，还设计了5色抗体组合对配对UA与切除组织中的T细胞、巨噬细胞及肿瘤细胞进行空间特征分析。结果：与切除样本来源的细胞相比，UA获取的细胞在产量、活性以及肿瘤浸润性T细胞、B细胞和NK淋巴细胞的丰度方面均表现出可比性。重要的是，我们观察到两种组织样本中T细胞的免疫检查点表达强度具有高度一致性。结论：这些发现证实了利用超声吸引术获取的胶质母细胞瘤组织进行免疫浸润研究的可行性与可靠性。
肿瘤(癌症)患者之家