Objectives: Head and neck squamous cell carcinoma (HNSCC) is characterized by complex genetic alterations. This study aimed to identifyWBP5as a promising therapeutic target and evaluate the effect ofWBP5expression on prognosis and treatment response in HNSCC. Methods: Publicly available datasets were comprehensively analyzed to investigateWBP5expression through comprehensive bioinformatics analysis and functional validation. Results:WBP5was particularly overexpressed in HNSCC, as analyzed through the Gene Expression Profiling Interactive Analysis version 2 (GEPIA2) database and validated using multiple Gene Expression Omnibus (GEO) datasets. Analysis with UALCAN confirmed thatWBP5expression was significantly higher in advanced cancer stages and tumor grades than that of normal samples. A Kaplan–Meier analysis demonstrated that patients overexpressingWBP5had a poor prognosis. Moreover, WBP5 expression correlated with the overexpression of the epidermal growth factor receptor in HNSCC. In vitro experiments revealed thatWBP5knockdown significantly reduced FaDu cell proliferation and viability. Furthermore, silencingWBP5enhanced cisplatin sensitivity, indicating its potential role in chemoresistance. Conclusions: These results indicate thatWBP5could act as a prognostic marker and a viable therapeutic target in HNSCC. Modulating WBP5 expression may represent a novel strategy to enhance treatment efficacy. Future studies should elucidate the precise mechanisms ofWBP5action and develop targeted therapies. This integrated approach, combining a comprehensive analysis of publicly available datasets with in vitro experimental validation provides strong evidence for the clinical significance ofWBP5in HNSCC.
目的：头颈部鳞状细胞癌（HNSCC）以复杂的遗传改变为特征。本研究旨在鉴定WBP5作为潜在治疗靶点，并评估WBP5表达对HNSCC预后及治疗反应的影响。方法：通过综合分析公共数据库，结合生物信息学分析与功能验证，系统研究WBP5的表达特征。结果：基于GEPIA2数据库分析发现WBP5在HNSCC中特异性高表达，该结果经多个GEO数据集验证。UALCAN分析证实，与正常样本相比，WBP5在晚期癌症分期和高级别肿瘤中表达显著升高。Kaplan-Meier分析显示WBP5过表达患者预后不良。此外，WBP5表达与HNSCC中表皮生长因子受体的过表达呈正相关。体外实验表明，敲低WBP5能显著抑制FaDu细胞增殖与活力。更重要的是，沉默WBP5可增强顺铂敏感性，提示其在化疗耐药中的潜在作用。结论：WBP5可作为HNSCC的预后标志物和有效治疗靶点。调控WBP5表达可能成为提高治疗效果的新策略。未来研究需阐明WBP5作用的具体机制并开发靶向疗法。这种整合公共数据集系统分析与体外实验验证的研究方法，为WBP5在HNSCC中的临床意义提供了有力证据。
WBP5Expression Influences Prognosis and Treatment Response in Head and Neck Squamous Cell Carcinoma
肿瘤(癌症)患者之家