文章：

在小鼠肺癌模型中，微束与细束放射疗法展现出卓越的抗肿瘤反应

Superior Anti-Tumor Response After Microbeam and Minibeam Radiation Therapy in a Lung Cancer Mouse Model

原文发布日期：1 January 2025

DOI: 10.3390/cancers17010114

类型: Article

开放获取: 是

英文摘要：

Objectives: The present study aimed to compare the tumor growth delay between conventional radiotherapy (CRT) and the spatially fractionated modalities of microbeam radiation therapy (MRT) and minibeam radiation therapy (MBRT). In addition, we also determined the influence of beam width and the peak-to-valley dose ratio (PVDR) on tumor regrowth. Methods: A549, a human non-small-cell lung cancer cell line, was implanted subcutaneously into the hind leg of female CD1-Foxn1numice. The animals were irradiated with sham, CRT, MRT, or MBRT. The spatially fractionated fields were created using two specially designed multislit collimators with a beam width of 50 μm and a center-to-center distance (CTC) of 400 μm for MRT and a beam width of 500 μm and 2000 μm CTC for MBRT. Additionally, the concept of the equivalent uniform dose (EUD) was chosen in our study. A dose of 20 Gy was applied to all groups with a PVDR of 20 for MBRT and MRT. Tumor growth was recorded until the tumors reached at least a volume that was at least three-fold of their initial value, and the growth delay was calculated. Results: We saw a significant reduction in tumor regrowth following all radiation modalities. A growth delay of 11.1 ± 8 days was observed for CRT compared to the sham, whereas MBRT showed a delay of 20.2 ± 7.3 days. The most pronounced delay was observed in mice irradiated with MRT PVDR 20, with 34.9 ± 26.3 days of delay. Conclusions: The current study highlights the fact that MRT and MBRT modalities show a significant tumor growth delay in comparison to CRT at equivalent uniform doses.

摘要翻译： 

目的：本研究旨在比较常规放疗（CRT）与空间分割放疗模式——微束放疗（MRT）及小束放疗（MBRT）在肿瘤生长延迟方面的差异。同时，我们还探讨了束流宽度及峰谷剂量比（PVDR）对肿瘤再生长的影响。方法：将人非小细胞肺癌细胞系A549皮下植入雌性CD1-Foxn1nu小鼠后肢。对实验动物分别实施假照射、CRT、MRT或MBRT照射。空间分割照射野通过两种特制多狭缝准直器实现：MRT采用束宽50μm、中心间距400μm的参数，MBRT采用束宽500μm、中心间距2000μm的参数。本研究采用等效均匀剂量（EUD）概念，所有组别均给予20Gy剂量，其中MBRT和MRT组的PVDR设定为20。持续记录肿瘤生长直至其体积达到初始值三倍以上，并计算生长延迟时间。结果：所有放疗模式均显著抑制肿瘤再生长。与假照射组相比，CRT组观察到11.1±8天的生长延迟，MBRT组延迟时间为20.2±7.3天。而接受PVDR=20的MRT照射的小鼠表现出最显著的延迟效应，延迟时间达34.9±26.3天。结论：本研究证实，在等效均匀剂量条件下，MRT与MBRT模式相较于CRT能产生更显著的肿瘤生长延迟效应。

原文链接：

Superior Anti-Tumor Response After Microbeam and Minibeam Radiation Therapy in a Lung Cancer Mouse Model