Background:Indoor radon is a significant risk factor for the development of LC. This study aimed to identify potential biomarkers for LC risk in high background radiation areas using a metabolomics approach (UHPLC-HRMS).Methods:Based on the indoor radon activity concentration measurements in the Kong Khaek subdistrict, serum samples were collected from 45 nonsmoker or former smoker participants, comprising 15 LC patients and 30 matched healthy controls (low- and high-radon groups, respectively).Results:A total of 90 and 111 differential metabolites were identified in the LC group compared with the low- and high-radon groups, respectively, using criteria such as a variable importance in projection (VIP) of >1, a fold change (FC) of >1 or <0.5, and apvalue of <0.05. Receiver operating characteristic (ROC) curves (an AUC of ≥ 0.9) indicated that 30 and 21 of these metabolites had the potential to serve as biomarkers of LC development in the low- and high-radon groups, respectively. The KEGG pathway enrichment analysis suggested that D-sphingosine may have been a candidate biomarker associated with LC in both groups.Conclusions:Overall, this study provides new insights into metabolic biomarkers for screening LC development in high-risk individuals with prolonged exposure to indoor radon. Further large-scale studies are needed to validate our results.
背景：室内氡是导致肺癌（LC）的重要风险因素。本研究旨在通过代谢组学方法（超高效液相色谱-高分辨质谱联用技术）识别高本底辐射地区肺癌风险的潜在生物标志物。 方法：基于孔凯县室内氡活度浓度测量结果，本研究采集了45名非吸烟或已戒烟参与者的血清样本，包括15名肺癌患者及30名匹配的健康对照者（分别对应低氡组与高氡组）。 结果：通过变量重要性投影值>1、倍数变化>1或<0.5及p值<0.05等标准，在肺癌组与低氡组、高氡组的比较中分别鉴定出90种和111种差异代谢物。受试者工作特征曲线（曲线下面积≥0.9）显示，其中分别有30种和21种代谢物具备作为低氡组与高氡组肺癌发生生物标志物的潜力。KEGG通路富集分析提示D-鞘氨醇可能是两组中均与肺癌相关的候选生物标志物。 结论：本研究为筛查长期暴露于室内氡的高危人群肺癌发生风险的代谢生物标志物提供了新见解，后续需开展大规模研究以验证结果。
肿瘤(癌症)患者之家