Background/Objective: Colorectal cancer (CRC) is one of the most common cancers worldwide. Increasing scientific evidence supports the idea that gut microbiota dysbiosis accompanies colorectal tumorigenesis, and these changes could be causative. Implementing gut microbiota analysis in clinical practice is limited by sample type, sequencing platform and taxonomic classification. This article aims to address these limitations, providing new insights into the microbiota associated with CRC pathogenesis and implementing its analyses in personalized medicine. Methods: To that aim, we evaluate differences in the bacterial composition of 130 paired tumor and non-tumor adjacent tissues from a cohort of CRC patients from the Biobank of the University of Navarra, Spain. The V3–V4 region of the 16S rRNA gene was amplified, sequenced using the MinION platform, and taxonomically classified using the NCBI database. Results: To our knowledge, this is the first study to report an increased relative abundance ofStreptococcus periodonticumand a decreased relative abundance ofCorynebacteriumassociated with CRC. Genera such asFusobacterium,LeptotrichiaandStreptococcusshowed higher relative abundances in tumor than in non-tumor tissues, as previously described in the literature. Specifically, we identified higher levels ofFusobacterium animalis,Fusobacterium nucleatum,Fusobacterium polymorphumandS. periodonticumin tumor tissues. In contrast, genera such asBacteroidesandCorynebacteriumshowed lower relative abundances in tumor tissues. There were also differences at the taxonomic level between tumor locations. Conclusions: These results, consistent with previous studies, further support the hypothesis thatLeptotrichiaandFusobacteriumcontribute to CRC progression, withF. nucleatumandF. animalisproposed as key CRC pathogenic taxa. Overall, these results contribute to a better understanding of the CRC-associated microbiota, addressing critical barriers to its implementation in personalized medicine.
背景/目的：结直肠癌是全球最常见的恶性肿瘤之一。越来越多的科学证据表明肠道菌群失调伴随结直肠肿瘤发生，且这种变化可能具有致病性。目前肠道菌群分析在临床实践中的应用受限于样本类型、测序平台和分类学方法。本文旨在突破这些局限，为结直肠癌发病机制相关的微生物群提供新见解，并推动其在个体化医疗中的应用。方法：为此，我们评估了来自西班牙纳瓦拉大学生物样本库的130对结直肠癌患者肿瘤组织与癌旁正常组织的细菌组成差异。通过扩增16S rRNA基因V3-V4区域，使用MinION平台进行测序，并基于NCBI数据库进行物种分类。结果：据我们所知，本研究首次报道了与结直肠癌相关的牙周链球菌相对丰度升高和棒状杆菌相对丰度降低。如文献所述，梭杆菌属、纤毛菌属和链球菌属在肿瘤组织中的相对丰度高于非肿瘤组织。具体而言，我们在肿瘤组织中检测到更高水平的动物梭杆菌、具核梭杆菌、多形梭杆菌和牙周链球菌。相反，拟杆菌属和棒状杆菌属在肿瘤组织中相对丰度较低。不同肿瘤部位在分类学水平上也存在差异。结论：这些与既往研究一致的结果进一步支持了纤毛菌属和梭杆菌属促进结直肠癌进展的假说，其中具核梭杆菌和动物梭杆菌被认为是关键的结直肠癌致病菌群。总体而言，本研究有助于深化对结直肠癌相关微生物群的认识，为克服其在个体化医疗应用中的关键障碍提供了新思路。
肿瘤(癌症)患者之家