Background: Ovarian carcinoma (OC) has an unfavorable prognosis due to lack of screening and an asymptomatic course. New diagnostic methods are being sought to enable earlier diagnosis of this condition. The purpose of this study was to determine the diagnostic utility of collagenases (MMP-1, MMP-8 and MMP-13) in the diagnosis of OC compared to HE4 and CA125 and the ROMA. Methods: The study group consisted of 120 patients with OC, the control group: 70 patients with benign ovarian lesions (BLs) and 50 healthy women (HS). MMP-1, MMP-8 and MMP-13 were determined by ELISA and HE4 and CA125 by CMIA. Results: OC patients had higher levels of MMP-1 and MMP-13 compared to the BL and HS groups. MMP-1 (SE: 81.66%; SP: 94%; PPV: 97.02%; NPV: 68.11%; AUC: 0.9625) and MMP-13 (SE: 77.50%; SP: 94%; PPV: 96.875%; NPV: 63.51%; AUC: 0.917) showed similar or higher diagnostic values to routine markers (HE4: SE:85%; SP: 92%; PPV: 96.22%; NPV: 71.875%; AUC: 0.943; CA125: SE: 80%; SP: 98%; PPV: 98.96%; NPV: 67.12%; AUC: 0.909) and the ROMA (SE: 90.83%; SP: 94%; PPV: 97.32%; NPV: 81.03%; AUC: 0.955). Performing combined analyses of individual MMPs and MMPs with ROMA was associated with further increases in diagnostic parameters. Conclusions: MMP-1 and MMP-13 have shown preliminary potential as diagnostic markers and auxiliary markers to ROMA in biochemical diagnosis of OC.
背景：卵巢癌（OC）因缺乏筛查手段且病程隐匿，预后较差。目前正在探索新的诊断方法以实现该疾病的早期诊断。本研究旨在评估胶原酶（MMP-1、MMP-8和MMP-13）相较于HE4、CA125及ROMA指数在卵巢癌诊断中的临床应用价值。方法：研究组纳入120例卵巢癌患者，对照组包括70例卵巢良性病变（BLs）患者及50例健康女性（HS）。采用ELISA法检测MMP-1、MMP-8和MMP-13水平，CMIA法检测HE4和CA125水平。结果：与BL组和HS组相比，卵巢癌患者MMP-1和MMP-13水平显著升高。MMP-1（灵敏度：81.66%；特异度：94%；阳性预测值：97.02%；阴性预测值：68.11%；AUC：0.9625）和MMP-13（灵敏度：77.50%；特异度：94%；阳性预测值：96.875%；阴性预测值：63.51%；AUC：0.917）的诊断效能与常规标志物相当或更优（HE4：灵敏度85%；特异度92%；阳性预测值96.22%；阴性预测值71.875%；AUC：0.943；CA125：灵敏度80%；特异度98%；阳性预测值98.96%；阴性预测值67.12%；AUC：0.909），且接近ROMA指数（灵敏度90.83%；特异度94%；阳性预测值97.32%；阴性预测值81.03%；AUC：0.955）。联合分析单一MMPs指标及MMPs与ROMA的组合可进一步提升诊断参数。结论：在卵巢癌生化诊断中，MMP-1和MMP-13显示出作为独立诊断标志物及ROMA指数辅助标志物的潜在价值。
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