Background: Cervical cancer is the fourth most common cancer among women worldwide. The zinc finger transcription factor 281 (ZNF281)/ZBP-99 protein specifically binds to GC-rich DNA sequences and regulates gene expression, and it has been shown to promote tumor progression. In this study, we aim to investigate the function and molecular mechanism of ZNF281 in uterine cervical carcinoma.Methods: We conducted immunohistochemistry and Western blot assays to determine the expression of ZNF281 in eight human cervical cancer tissues. And, xenograft experiments involving the injection of HeLa cells into nude mice was used to determine the function of ZNF281 on proliferation. Transwell assays were used to detect the migration and invasion of HeLa cells after indicated that ZNF281 overexpression.Results:Our results indicated that ZNF281 protein levels were higher in cervical cancer tissues compared to normal cervical tissues. Additionally, ZNF281 was expressed in human cervical carcinoma cell lines, including HeLa, SiHa, C-33 A, CaSki, and HT-3, and is localized in both the cell nucleus and cytoplasm. ZNF281 overexpression did not influence HeLa cell proliferation or tumor size in situ. Moreover, nude mice injected with ZNF281-overexpressing cell lines developed more tumor lesions in the lungs compared to those injected with control cell lines.Conclusions: These findings suggest that ZNF281 is associated with tumor metastasis without affecting cell proliferation, both in vivo and in vitro.
背景：宫颈癌是全球女性第四大常见癌症。锌指转录因子281（ZNF281）/ZBP-99蛋白特异性结合富含GC的DNA序列并调控基因表达，已被证实可促进肿瘤进展。本研究旨在探讨ZNF281在子宫颈癌中的功能及分子机制。 方法：采用免疫组化与蛋白质印迹法检测八例人宫颈癌组织中ZNF281的表达水平。通过将HeLa细胞注射至裸鼠体内的异种移植实验，验证ZNF281对增殖功能的影响。采用Transwell实验检测ZNF281过表达后HeLa细胞的迁移与侵袭能力。 结果：研究显示宫颈癌组织中ZNF281蛋白水平显著高于正常宫颈组织。ZNF281在HeLa、SiHa、C-33 A、CaSki及HT-3等人宫颈癌细胞系中均有表达，并定位于细胞核与细胞质。ZNF281过表达未影响HeLa细胞增殖能力及原位肿瘤体积。值得注意的是，注射ZNF281过表达细胞系的裸鼠较对照组在肺部形成更多肿瘤病灶。 结论：本研究表明ZNF281在体内外均与肿瘤转移相关，但不影响细胞增殖过程。
ZNF281 Facilitates the Invasion of Cervical Cancer Cell Both In Vivo and In Vitro
肿瘤(癌症)患者之家