Background/Objectives: The aim of this study was to examine the expression of TYK2 in colorectal cancer (CRC) and to determine the potential diagnostic and prognostic significance of this kinase. Methods: Digital image analysis was performed to assess immunohistochemical TYK2 reactivity. Results: There were significant differences for all positive pixels between CRC and normal colonic mucosa, with higher TYK2 expression levels observed in surgical margins than in adenocarcinomas (p= 0.0004). Pairedttests showed elevated immunoreactivity for overall TYK2 expression in matched pairs of CRC with adjacent surgical margins (p< 0.0001). Higher percentages of weak (p< 0.0001) and strong pixels (p= 0.0260) were detected in normal colonic mucosa than in cancer tissues. To distinguish cancer from normal intestinal mucosa, the following cutoffs for the TYK2 immune score were found: 29.5% for all cases and 31% for matched pairs. Tumor budding (Bd) was negatively correlated with the percentage of strong pixels for TYK2 (ρ = −0.270,p= 0.0096). The percentage of strong pixels was significantly elevated for the T parameter (p= 0.0428). There was a positive correlation between the number of involved lymph nodes and weak pixels (ρ = 0.239,p= 0.0242). Immunofluorescence staining showed significantly higher signal intensities in colonic mucosa than in CRC. The protein level of TYK2 was significantly higher in controls than in cancer tissues. TEM imaging showed lower levels of TYK2 in cancer than in ulcerative colitis. Conclusions: TYK2 protein expression may bring diagnostic value in patients diagnosed with CRC.
背景/目的：本研究旨在探讨TYK2在结直肠癌（CRC）中的表达情况，并评估该激酶潜在的诊断及预后意义。方法：采用数字图像分析技术评估免疫组化TYK2反应性。结果：结直肠癌组织与正常结肠黏膜在所有阳性像素点均存在显著差异，手术切缘组织的TYK2表达水平显著高于腺癌组织（p=0.0004）。配对t检验显示，在匹配的结直肠癌与邻近手术切缘组织对中，TYK2总体表达的免疫反应性显著升高（p<0.0001）。正常结肠黏膜中弱阳性（p<0.0001）与强阳性像素点（p=0.0260）占比均显著高于癌组织。为区分癌组织与正常肠黏膜，确定TYK2免疫评分临界值为：全部病例29.5%，配对病例31%。肿瘤出芽（Bd）与TYK2强阳性像素百分比呈负相关（ρ=-0.270，p=0.0096）。T分期参数中强阳性像素百分比显著升高（p=0.0428）。淋巴结转移数量与弱阳性像素呈正相关（ρ=0.239，p=0.0242）。免疫荧光染色显示结肠黏膜信号强度显著高于结直肠癌组织。对照组TYK2蛋白水平显著高于癌组织。透射电镜成像显示癌组织中TYK2水平低于溃疡性结肠炎。结论：TYK2蛋白表达可能为结直肠癌患者的诊断提供临床价值。
肿瘤(癌症)患者之家