文章：

G蛋白偶联受体相关分选蛋白1的过表达与良性前列腺增生、早期前列腺恶性疾病及前列腺癌的进展相关

G-Protein-Coupled Receptor-Associated Sorting Protein 1 Overexpression Is Involved in the Progression of Benign Prostatic Hyperplasia, Early-Stage Prostatic Malignant Diseases, and Prostate Cancer

原文发布日期：30 October 2024

DOI: 10.3390/cancers16213659

类型: Article

开放获取: 是

英文摘要：

Background/Objectives:Prostate cancer (PCa) is a prevalent malignancy, necessitating accurate diagnostic methods to distinguish it from benign conditions such as benign prostatic hyperplasia (BPH). Current diagnostic tools, relying primarily on serum prostate-specific antigen (PSA) levels, lack specificity, leading to an over-diagnosis and unnecessary treatment of patients with benign conditions. This study explores G-protein-coupled receptor-associated sorting protein 1 (GASP-1) as a more sensitive biomarker for PCa detection.Methods:Prostate tissue microarrays of healthy, BPH, and prostate cancer patients with different Gleason scores were studied. Polyclonal antibodies targeted against GASP-1 were used for routine immunohistochemistry (IHC) and enzyme-linked immunosorbent assay (ELISA) analyses.Results:The results indicated a 5-fold difference in serum GASP-1 levels between BPH and PCa, which was validated through GASP-1 IHC. Furthermore, a novel scoring system, the H-score, assesses GASP-1 granules’ intensity and size, revealing a clear distinction between BPH and PCa. An additional analysis of GASP-1 expression between PCa cases with different Gleason scores reveals that GASP-1 overexpression correlates with PCa severity, providing insights into disease progression.Conclusions: The study supports GASP-1′s role as a promising diagnostic marker, supplementing PSA testing, and offering improved risk stratification for PCa. Additionally, an open-source software system is introduced for an efficient GASP-1 granule color analysis, enhancing diagnostic accuracy.

摘要翻译： 

背景/目的：前列腺癌（PCa）是一种常见的恶性肿瘤，需要准确的诊断方法来区分其与良性前列腺增生（BPH）等良性病变。目前主要依赖血清前列腺特异性抗原（PSA）水平的诊断工具缺乏特异性，导致对良性病变患者的过度诊断和不必要治疗。本研究探讨了G蛋白偶联受体相关分选蛋白1（GASP-1）作为前列腺癌检测中更敏感的生物标志物。 方法：研究使用了健康人群、BPH患者以及具有不同Gleason评分的前列腺癌患者的前列腺组织微阵列。采用针对GASP-1的多克隆抗体进行常规免疫组织化学（IHC）和酶联免疫吸附测定（ELISA）分析。 结果：结果显示，BPH与前列腺癌患者血清GASP-1水平存在5倍差异，这一结果通过GASP-1免疫组化得到验证。此外，一种新的评分系统——H评分，评估了GASP-1颗粒的强度和大小，揭示了BPH与前列腺癌之间的明显区别。对不同Gleason评分前列腺癌病例中GASP-1表达的进一步分析表明，GASP-1过表达与前列腺癌严重程度相关，为疾病进展提供了见解。 结论：本研究支持GASP-1作为一种有前景的诊断标志物，可补充PSA检测，并为前列腺癌提供改进的风险分层。此外，引入了一种开源软件系统，用于高效进行GASP-1颗粒颜色分析，从而提高诊断准确性。

原文链接：

G-Protein-Coupled Receptor-Associated Sorting Protein 1 Overexpression Is Involved in the Progression of Benign Prostatic Hyperplasia, Early-Stage Prostatic Malignant Diseases, and Prostate Cancer