Background:Cervical cancer is fourth the most common cancer in women worldwide. Due to the prevalence of human papillomavirus (HPV) in the population (80–90%), scientists are likely to discover even more associations of this pathogen with other diseases in the future. In recent years, In Situ Hybridization (ISH) assays that use automated signal-detecting methods in formalin-fixed, paraffin-embedded (FFPE) cervical tissue, such as the enzyme-categorized signal-detecting system, have shown a higher sensitivity.Objectives and Methods:To evaluate automatic signal detection of ISH assay for detecting HPV DNA, we compared the ability of an ISH probe, Inform HPV II and III (Ventana Medical Systems, Tucson, AZ), to that of PCR assays to detect HPV DNA in cervical tissue specimens with cervical intraepithelial neoplasia (CIN; CIN 1, 28 cases; CIN 2, 22 cases; and CIN 3, 20 cases) and normal cervix (2 cases).Results:Our findings showed a significant relation was confirmed between ISH III level and HPV outcome (positive/negative). Patients with positive HPV outcomes had significantly lower ISH III levels, MD = −7961.82 CI95[−17,230.00; −199.21],p= 0.005.Conclusions:Automatic signal detection of ISH assay is not particularly applicable to cervical tissue material. A more useful method of confirming the presence of HPV in the cervix is the HPV test with genotyping, as it allows for collecting a larger amount of material from the cervical disc and canal. The interpretation of a positive or negative ISH test must be guided in the context of clinical history and morphology.
背景：宫颈癌是全球女性第四大常见癌症。由于人乳头瘤病毒（HPV）在人群中的高流行率（80-90%），科学家未来很可能发现该病原体与其他疾病的更多关联。近年来，在福尔马林固定、石蜡包埋（FFPE）的宫颈组织中采用自动化信号检测方法（如酶分类信号检测系统）的原位杂交（ISH）检测显示出更高的灵敏度。 目的与方法：为评估ISH检测自动信号检测系统对HPV DNA的检测能力，我们比较了Inform HPV II和III型ISH探针（Ventana Medical Systems, Tucson, AZ）与PCR检测在宫颈组织样本中检测HPV DNA的能力。样本包括宫颈上皮内瘤变（CIN；CIN 1级28例，CIN 2级22例，CIN 3级20例）及正常宫颈组织（2例）。 结果：研究证实ISH III水平与HPV检测结果（阳性/阴性）存在显著相关性。HPV阳性患者的ISH III水平显著降低，平均差MD = -7961.82，95%置信区间CI95[-17,230.00; -199.21]，p=0.005。 结论：ISH检测的自动信号检测方法在宫颈组织材料中适用性有限。确认宫颈HPV感染的更有效方法是结合基因分型的HPV检测，该方法可从宫颈表面及宫颈管获取更充足的检测材料。ISH检测阳性或阴性结果的解读必须结合临床病史和组织形态学特征进行综合判断。
肿瘤(癌症)患者之家