文章：

宫颈镜检查后组织学结果中14种高危HPV亚型分布及p16/Ki67双染状态分析：阴性、低级别与高级别宫颈鳞状上皮内病变

Distribution of 14 High-Risk HPV Types and p16/Ki67 Dual-Stain Status in Post-Colposcopy Histology Results: Negative, Low- and High-Grade Cervical Squamous Intraepithelial Lesions

原文发布日期：5 October 2024

DOI: 10.3390/cancers16193401

类型: Article

开放获取: 是

英文摘要：

Background: Determining the distribution of high-risk human papillomavirus (HR-HPV) types in histologic low-(LSIL) and high-grade (HSIL/CIN2+) squamous intraepithelial lesions through a diagnostic process in a cervical cancer prevention provides one of the key etiological factors behind further progression and persistence. Incorporating novel high-grade cervical lesion biomarkers such as p16/Ki67 dual staining (DS) alongside HPV typing has become important in detecting cervical precancers. Methods: Among 28,525 screening tests and 602 histology results, 559 cases with HR-HPV and histology results obtained from colposcopic biopsy were retrospectively analyzed, together with DS status. The χ2test with Bonferroni correction evaluated the differences in HR-HPV type prevalence and DS positivity across three histologic study groups. Results: A statistically significant difference in the prevalence of HPV 16 was observed between negative and HSIL/CIN2+ (p= 0.00027) groups, as well as between the LSIL/CIN1 and HSIL/CIN2+ groups (p= 0.00041). However, no significant difference was found between the negative and LSIL/CIN1 groups. Similarly, the DS positivity difference was significant between the negative and HSIL/CIN2+ (p< 0.0001) and between the LSIL/CIN1 and HSIL/CIN2+ groups (p< 0.0001), but there was no significant difference between the negative and LSIL/CIN1 groups. Conclusions: The study highlights the heterogeneous nature of HPV-related cervical pathologies, and the distinct risks associated with different cervical lesion grades, emphasizing the importance of HR-HPV type distribution and DS status.

摘要翻译： 

背景：在宫颈癌预防的诊断过程中，确定高危型人乳头瘤病毒（HR-HPV）在组织学低级别（LSIL）和高级别（HSIL/CIN2+）鳞状上皮内病变中的分布，是揭示病变持续进展的关键病因因素之一。将新型高级别宫颈病变生物标志物如p16/Ki67双重染色（DS）与HPV分型相结合，对于宫颈癌前病变的检测具有重要意义。方法：在28,525例筛查检测和602例组织学结果中，回顾性分析了559例经阴道镜活检获得HR-HPV和组织学结果并同时具备DS状态的病例。采用经Bonferroni校正的χ2检验评估了三个组织学研究组之间HR-HPV型别流行率和DS阳性率的差异。结果：在阴性组与HSIL/CIN2+组之间（p=0.00027），以及LSIL/CIN1组与HSIL/CIN2+组之间（p=0.00041），观察到HPV 16的流行率存在统计学显著差异。然而，阴性组与LSIL/CIN1组之间未发现显著差异。同样，DS阳性率在阴性组与HSIL/CIN2+组之间（p<0.0001）以及LSIL/CIN1组与HSIL/CIN2+组之间（p<0.0001）差异显著，但阴性组与LSIL/CIN1组之间无显著差异。结论：本研究强调了HPV相关宫颈病变的异质性，以及不同级别宫颈病变相关的独特风险，凸显了HR-HPV型别分布和DS状态的重要性。

原文链接：

Distribution of 14 High-Risk HPV Types and p16/Ki67 Dual-Stain Status in Post-Colposcopy Histology Results: Negative, Low- and High-Grade Cervical Squamous Intraepithelial Lesions