文章：

在结肠癌原位小鼠模型中，肝转移灶荧光抗CEA抗体靶向与荧光素酶表达的精准共定位

Accurate Co-Localization of Luciferase Expression and Fluorescent Anti-CEA Antibody Targeting of Liver Metastases in an Orthotopic Mouse Model of Colon Cancer

原文发布日期：29 September 2024

DOI: 10.3390/cancers16193341

类型: Article

开放获取: 是

英文摘要：

Background: The present study aimed to validate the accuracy of a tumor-specific antibody to target liver metastases of colorectal cancer. Methods: A humanized anti-CEA antibody conjugated to a fluorescent dye (M5A-IR800) was tested for targeting human colorectal cancer liver metastases (CRLMs) expressing luciferase in an orthotopic mouse model. Orthotopic mouse models of CRLMs were established by implanting fragments of a luciferase-expressing human colorectal cancer cell line, LS174T, in the liver of nude mice. Mice received 50 µg M5A-IR800 72 h prior to imaging. To test co-localization, bioluminescence imaging was performed using D-luciferin, which was given via intraperitoneal injection just prior to imaging. Results: Tumors were able to be visualized non-invasively through the skin with the luciferase–luciferin signal. Intra-abdominal imaging showed accurate labeling of CRLMs with M5A-IR800, which co-localized with the luciferase–luciferin signal. Conclusions: The present results validate the accuracy of a tumor-specific anti-CEA antibody in targeting liver metastases of colorectal cancer.

摘要翻译： 

背景：本研究旨在验证一种肿瘤特异性抗体靶向结直肠癌肝转移灶的准确性。方法：在荷瘤小鼠原位模型中，测试了与荧光染料（M5A-IR800）偶联的人源化抗CEA抗体靶向表达荧光素酶的人结直肠癌肝转移灶（CRLMs）的能力。通过将表达荧光素酶的人结直肠癌细胞系LS174T的组织片段植入裸鼠肝脏，建立CRLMs原位小鼠模型。小鼠在成像前72小时接受50 µg M5A-IR800注射。为验证共定位效果，在成像前通过腹腔注射D-荧光素进行生物发光成像。结果：通过荧光素酶-荧光素信号可无创地经皮肤观察到肿瘤。腹腔内成像显示M5A-IR800能准确标记CRLMs，且与荧光素酶-荧光素信号实现共定位。结论：本研究结果验证了肿瘤特异性抗CEA抗体靶向结直肠癌肝转移灶的准确性。

原文链接：

Accurate Co-Localization of Luciferase Expression and Fluorescent Anti-CEA Antibody Targeting of Liver Metastases in an Orthotopic Mouse Model of Colon Cancer