Backgroung: The rising complexity and associated side effects of cancer treatments highlight the need for safer and more effective therapeutic agents. Carbon-based nanomaterials such as CDs have been gaining prominence for their unique characteristics, opening avenues for diverse applications such as fluorescence imaging, drug and gene transport, controlled drug delivery, medical diagnosis, and biosensing. Despite promising advancements in research, it remains imperative to scrutinize the properties and potential cytotoxicity of newly developed CDs, ensuring their viability for these applications. Methods: We synthesized four N-doped CDs through a hydrothermal method. Cell viability assays were conducted on A549 and UM-UC-5 cancer cells at a range of concentrations and incubation times, both individually and with the chemotherapeutic agent 5-fluorouracil (5-FU). Results: The obtained results suggest that the newly developed CDs exhibit suitability for applications such as bioimaging, as no significant impact on cell viability was observed for CDs alone.
背景：癌症治疗日益复杂及其伴随的副作用凸显了对更安全、更有效治疗药物的需求。碳基纳米材料如碳点（CDs）因其独特性质而备受关注，为荧光成像、药物与基因递送、可控药物释放、医学诊断及生物传感等多样化应用开辟了新途径。尽管研究已取得显著进展，但深入评估新型碳点的特性及其潜在细胞毒性仍至关重要，以确保其在上述应用中的可行性。 方法：本研究通过水热法合成了四种氮掺杂碳点。在多种浓度及孵育时间条件下，分别对A549和UM-UC-5癌细胞单独使用碳点，以及联合化疗药物5-氟尿嘧啶（5-FU）进行了细胞活力检测。 结果：实验结果表明，新型碳点适用于生物成像等应用领域，因为单独使用碳点未观察到对细胞活力产生显著影响。
Safety Evaluation of Carbon Dots in UM-UC-5 and A549 Cells for Biomedical Applications
肿瘤(癌症)患者之家