Sporadic vestibular schwannomas (VSs) often exhibit slow or negligible growth. Nevertheless, some VSs increase significantly in volume within a few months or grow continuously. Recent evidence indicates a role of inflammation in promoting VS growth. Therefore, our study aimed to identify cytokines, which are associated with larger VSs. The expression of different cytokines in VS tumor samples and VS primary cultures was investigated. Additionally, the concentration of cytokines in cell culture supernatants of VS primary cultures and cerebrospinal fluid (CSF) of VS patients and healthy controls were determined. Correlation analysis of cytokine levels with tumor volume, growth rate, Koos grade, age, and hearing was examined with Spearman’s-rank test. The mRNA expression of CC-chemokine ligand (CCL) 18, growth differentiation factor (GDF) 15, and interferon regulatory factor 4 correlated positively with tumor volume. Moreover, the amount of GDF15 in the cell culture supernatant of primary cells correlated positively with tumor volume. The concentrations of the cytokines CCL2, CCL5, and CCL18 and transforming growth factor beta (TGFB) 1 in the CSF of the patients were significantly different from those in the CSF controls. Inhibition of immune cell infiltration could be a putative approach to prevent and control VS growth.
散发性前庭神经鞘瘤（VSs）通常表现出缓慢或可忽略的生长。然而，部分VSs在数月内体积显著增大或持续生长。近期证据表明炎症在促进VS生长中发挥作用。因此，本研究旨在鉴定与较大体积VS相关的细胞因子。研究检测了VS肿瘤样本及VS原代培养物中不同细胞因子的表达水平，并测定了VS原代培养细胞上清液、VS患者及健康对照者脑脊液（CSF）中的细胞因子浓度。采用斯皮尔曼秩相关检验分析细胞因子水平与肿瘤体积、生长速率、Koos分级、年龄及听力之间的相关性。结果显示，CC趋化因子配体（CCL）18、生长分化因子（GDF）15及干扰素调节因子4的mRNA表达与肿瘤体积呈正相关。此外，原代细胞培养上清液中GDF15的含量与肿瘤体积呈正相关。患者CSF中细胞因子CCL2、CCL5、CCL18及转化生长因子β（TGFB）1的浓度与对照组CSF存在显著差异。抑制免疫细胞浸润可能是预防和控制VS生长的潜在策略。
肿瘤(癌症)患者之家